Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/5162
Título
Low-Cost Optical Mapping Systems for Panoramic Imaging of Complex Arrhythmias and Drug-Action in Translational Heart Models
Autor(es)
Lee, Peter | Calvo, Conrado J. CNIC | Alfonso-Almazan, Jose M. CNIC | Quintanilla, Jorge G. CNIC | Chorro, Francisco J. | Yan, Ping | Loew, Leslie M. | Filgueiras-Rama, David CNIC | Millet, Jose
Fecha de publicación
2017
Cita
Sci Rep. 2017; 7:43217
Idioma
Inglés
Tipo de documento
journal article
Resumen
Panoramic optical mapping is the primary method for imaging electrophysiological activity from the entire outer surface of Langendorff-perfused hearts. To date, it is the only method of simultaneously measuring multiple key electrophysiological parameters, such as transmembrane voltage and intracellular free calcium, at high spatial and temporal resolution. Despite the impact it has already had on the fields of cardiac arrhythmias and whole-heart computational modeling, present-day system designs precludes its adoption by the broader cardiovascular research community because of their high costs. Taking advantage of recent technological advances, we developed and validated low-cost optical mapping systems for panoramic imaging using Langendorff-perfused pig hearts, a clinically-relevant model in basic research and bioengineering. By significantly lowering financial thresholds, this powerful cardiac electrophysiology imaging modality may gain wider use in research and, even, teaching laboratories, which we substantiated using the lower-cost Langendorff-perfused rabbit heart model.
Palabras clave
3-DIMENSIONAL SURFACE RECONSTRUCTION | PERSISTENT ATRIAL-FIBRILLATION | VENTRICULAR-FIBRILLATION | CARDIAC ELECTROPHYSIOLOGY | EPICARDIAL ACTIVATION | ACTION-POTENTIALS | RABBIT HEART | VOLTAGE | TACHYCARDIA | MECHANISMS
Versión en línea
DOI
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