Mostrar el registro sencillo del ítem

dc.contributor.authorPontes-Quero, Samuel 
dc.contributor.authorHeredia, Luis 
dc.contributor.authorCasquero-Garcia, Veronica 
dc.contributor.authorFernandez-Chacon, Macarena 
dc.contributor.authorLuo, Wen 
dc.contributor.authorHermoso, Ana 
dc.contributor.authorBansal, Mayank 
dc.contributor.authorGarcia-Gonzalez, Irene 
dc.contributor.authorSanchez-Munoz, Maria S. 
dc.contributor.authorPerea, Juan R. 
dc.contributor.authorGaliana-Simal, Adrian 
dc.contributor.authorRodriguez-Arabaolaza, Iker 
dc.contributor.authorDel Olmo-Cabrera, Sergio 
dc.contributor.authorRocha, Susana 
dc.contributor.authorCriado-Rodriguez, Luis M. 
dc.contributor.authorGiovinazzo, Giovanna 
dc.contributor.authorBenedito, Rui 
dc.date.accessioned2017-10-20T10:23:09Z
dc.date.available2017-10-20T10:23:09Z
dc.date.issued2017
dc.identifierISI:000407445700019
dc.identifier.citationCell. 2017; 170(4):800-814 e18
dc.identifier.issn0092-8674
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5100
dc.description.abstractImproved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue. Here, we describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution.
dc.description.sponsorshipThis work was supported by grants to the PI R.B. from the Spanish Ministry of Economy, Industry and Competitiveness (SAF2013-44329-P, SAF2013-42359-ERC, and RYC-2013-13209) and European Research Council (ERC-2014-StG - 638028). S.P.-Q., M.F.-C., and I.G.-G. were supported by PhD fellowships from Fundacion La Caixa (CX-SO-2013-02, CX\_E-2015-01, and CX-SO-16-1, respectively). W.L. by a FP7-PEOPLE-2012-COFUND GA600396 postdoctoral contract. We thank Simon Bartlett for English editing, Ralf H. Adams for sharing the Cdh5(PAC)-CreERT2 mice, Jose Luis de La Pompa for comments throughout the project and for sharing the Tie2-Cre mice, Gonzalo Gancedo for the help with the mouse colony, Valeria Caiolfa for the help with the microscopy, and all the members of the CNIC gene targeting, transgenesis, cellomics, and microscopy units. The CNIC is supported by MEIC/MINECO and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505).
dc.language.isoeng
dc.publisherCell Press 
dc.type.hasVersionVoR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCRE-LOXP
dc.subjectIN-VIVO
dc.subjectMOUSE
dc.subjectMICE
dc.subjectEXPRESSION
dc.subjectRECOMBINATION
dc.subjectSYSTEM
dc.titleDual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID28802047
dc.format.volume170
dc.format.page800+
dc.identifier.doi10.1016/j.cell.2017.07.031
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC) 
dc.contributor.funderFundación La Caixa 
dc.contributor.funderFundación ProCNIC 
dc.description.peerreviewed
dc.identifier.e-issn1097-4172
dc.relation.publisherversionhttps://doi.org/10.1016/j.cell.2017.07.031
dc.identifier.journalCell
dc.repisalud.orgCNICCNIC::Grupos de investigación::Genética Funcional del Sistema de Fosforilación Oxidativa
dc.repisalud.orgCNICCNIC::Unidades técnicas::Transgénesis
dc.repisalud.orgCNICCNIC::Unidades técnicas::Células Pluripotentes
dc.repisalud.institucionCNIC
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-44329-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-42359-ERCes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2013-13209es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/600396/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/638028/EUes_ES
dc.rights.accessRightsopen accesses_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional