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dc.contributor.authorMartínez-Orellana, Pamela
dc.contributor.authorMartorell, Jaume
dc.contributor.authorVidaña, Beatriz
dc.contributor.authorMajó, Natalia
dc.contributor.authorMartínez, Jorge
dc.contributor.authorFalcon, Ana
dc.contributor.authorRodriguez-Frandsen, Ariel
dc.contributor.authorCasas Flecha, Inmaculada 
dc.contributor.authorPozo Sanchez, Francisco 
dc.contributor.authorGarcía-Migura, Lourdes
dc.contributor.authorGarcia-Barreno, Blanca 
dc.contributor.authorMelero, Jose Antonio 
dc.contributor.authorFraile, Lorenzo 
dc.contributor.authorNieto, Amelia
dc.contributor.authorMontoya, Maria
dc.identifier.citationVirol J. 2015; 12:48
dc.description.abstractBACKGROUND: The majority of pandemic 2009 H1N1 (A(H1N1)pdm09) influenza virus (IV) caused mild symptoms in most infected patients, however, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. The purpose of this work was to study in ferrets the dynamics of infection of two contemporary strains of human A(H1N1)pdm09 IV, one isolated from a patient showing mild disease and the other one from a fatal case. METHODS: Viral strains isolated from a patient showing mild disease-M (A/CastillaLaMancha/RR5661/2009) or from a fatal case-F (A/CastillaLaMancha/RR5911/2009), both without known comorbid conditions, were inoculated in two groups of ferrets and clinical and pathological conditions were analysed. RESULTS: Mild to severe clinical symptoms were observed in animals from both groups. A clinical score distribution was applied in which ferrets with mild clinical signs were distributed on a non-severe group (NS) and ferrets with severe clinical signs on a severe group (S), regardless of the virus used in the infection. Animals on S showed a significant decrease in body weight compared to animals on NS at 4 to 7 days post-infection (dpi). Clinical progress correlated with histopathological findings. Concentrations of haptoglobin (Hp) and serum amyloid A (SAA) increased on both groups after 2 dpi. Clinically severe infected ferrets showed a stronger antibody response and higher viral titres after infection (p = 0.001). CONCLUSIONS: The severity in the progress of infection was independent from the virus used for infection suggesting that the host immune response was determinant in the outcome of the infection. The diversity observed in ferrets mimicked the variability found in the human population.
dc.description.sponsorshipThe authors kindly thank Dr. Juan Ortín for his scientific contribution. This work was partially supported by Instituto de Salud Carlos III (Programa especial de investigación sobre la gripe pándemica GR09/0023, GR09/0040, GR09/0039), AGL2013-48923-C2-02 and CIBER de Enfermedades Infecciosas.
dc.publisherBioMed Central (BMC) 
dc.subjectPandemic A(H1N1)pdm09
dc.subjectClinical response
dc.titleClinical response to pandemic h1n1 influenza virus from a fatal and mild case in ferrets
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.identifier.journalVirology Journal
dc.repisalud.centroISCIII::Centro Nacional de Microbiología
dc.rights.accessRightsopen accesses_ES

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