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dc.contributor.authorBerzosa, Pedro 
dc.contributor.authorMula Lozano, Patricia 
dc.contributor.authorRincón, Jose Manuel Ramos
dc.contributor.authorGarcia, Luz 
dc.contributor.authorReyes, Francisco
dc.contributor.authorBenito, Agustin
dc.identifier.citationMalar J. 2015;14:357
dc.description.abstractBACKGROUND: Approximately 50 million people (60 %) live in malaria risk areas in Ethiopia, at altitudes below 2000 m. According to official data, 60-70 % of malaria cases are due to Plasmodium falciparum, and 40-30 % by Plasmodium vivax. The species Plasmodium ovale was detected in 2013 in the northwest of the country, being the first report of the presence of this species in Ethiopia since the 60 s. The aim of this study was to assess the diagnosis by microscopy and PCR, and demonstrate the presence of other Plasmodium species in the country. METHODS: The survey was conducted in Bulbula, situated in the Rift Valley (West Arsi Province, Oromia Region). From December 2010 to October 2011, 3060 samples were collected from patients with symptoms of malaria; the diagnosis of malaria was done by microscopy and confirmation by PCR. RESULTS: 736 samples were positive for malaria by microscopy. After removing the 260 samples (109 positives and 151 negatives) for which it was not possible to do PCR, there were a total of 2800 samples, 1209 are used for its confirmation by PCR and quality control (627 are positives and 582 negatives by microscopy). From the 627 positive samples, 604 were confirmed as positive by PCR, 23 false positives were detected, and the group of 582 negative samples, 184 were positive by PCR (false negatives), which added to the previous positive samples is a total of 788, positive samples for some species of Plasmodium sp. 13.3 % more positives were detected with the PCR than the microscopy. Importantly, 23 samples were detected by PCR as P. ovale, after the sequencing of these samples was determined as P. ovale curtisi. CONCLUSIONS: The PCR detected more positive samples than the microscopy; in addition, P. ovale and P. ovale/P. vivax were detected that had not been detected by microscopy, which can affect in the infection control.
dc.description.sponsorshipWe would like to thank the laboratory staff of the Bulbula Health Centre for their assistance in collecting the data, the nursing staff for their care of the patients and the Network of Tropical Diseases Research Centres (Red de Investigación Cooperativa en Enfermedades Tropicales/RICET—RD06/0021/0000). This study (Project Reference TRPY 1400/09) was funded by the Carlos III Institute of Health. I would like to acknowledge the help of Jose Miguel Rubio PhD (National Centre of Microbiology of the Institute of Health Carlos III) and to Maria Romay (National Centre for Tropical Medicine Institute of Health Carlos III).
dc.publisherBioMed Central
dc.relation.isversionofPublisher's version
dc.subjectMicroscopy diagnoses
dc.subjectP. ovale
dc.subjectPCR diagnoses
dc.titleQuality of malaria diagnosis and molecular confirmation of Plasmodium ovale curtisi in a rural area of the southeastern region of Ethiopia
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderRed de Investigación Cooperativa en Enfermedades Tropicales (España)
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.identifier.journalMalaria Journal
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropical

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Atribución 4.0 Internacional
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