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dc.contributor.authorVillalon-Panzano, Pilar 
dc.contributor.authorValdezate, Sylvia 
dc.contributor.authorCabezas, Teresa
dc.contributor.authorOrtega, Montserrat 
dc.contributor.authorGarrido, Noelia 
dc.contributor.authorVindel, Ana 
dc.contributor.authorMedina-Pascual, Maria Jose 
dc.contributor.authorSaez-Nieto, Juan A 
dc.date.accessioned2017-09-04T16:27:15Z
dc.date.available2017-09-04T16:27:15Z
dc.date.issued2015-02-25
dc.identifier.citationBMC Microbiol. 2015; 15: 47
dc.identifier.urihttp://hdl.handle.net/20.500.12105/4805
dc.description.abstractBACKGROUND: Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period. RESULTS: PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively. CONCLUSIONS: PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.
dc.language.isoeng
dc.publisherBioMed Central
dc.relation.isversionofPublisher's version
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectA. baumannii
dc.subjectPFGE
dc.subjectSequence type
dc.subjectMLVA
dc.subjectClone
dc.titleEndemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital
dc.typeArtículo
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID25887224
dc.format.volume15
dc.format.number1
dc.format.page47
dc.identifier.doi10.1186/s12866-015-0383-y
dc.description.peerreviewed
dc.identifier.e-issn1471-2180
dc.relation.publisherversionhttps://doi.org/10.1186/s12866-015-0383-y
dc.identifier.journalBMC Microbiology
dc.repisalud.centroISCIII::Centro Nacional de Microbiología::Área de Bacteriología, Micología Y Parasitología::Servicio de Bacteriología::Unidad de Taxonomía
dc.repisalud.institucionISCIII
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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