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dc.contributor.authorCunha, Joana
dc.contributor.authorCarrillo, Eugenia 
dc.contributor.authorSanchez Herrero, Carmen 
dc.contributor.authorCruz, Israel 
dc.contributor.authorMoreno, Javier 
dc.contributor.authorCordeiro-da-Silva, Anabela
dc.date.accessioned2017-09-04T16:25:27Z
dc.date.available2017-09-04T16:25:27Z
dc.date.issued2013-04-26
dc.identifier.citationParasit Vectors. 2013; 6: 122
dc.identifier.urihttp://hdl.handle.net/20.500.12105/4794
dc.description.abstractBACKGROUND: Leishmaniasis is a group of diseases with a variety of clinical manifestations. The form of the disease is highly dependent on the infective Leishmania species and the immunological status of the host. The infectivity of the parasite strain also plays an important role in the progression of the infection. The aim of this work is to understand the influence of the natural infectivity of Leishmania strains in the outcome of visceral leishmaniasis. METHODS: In this study we have characterized four strains of L. infantum in terms of molecular typing, in vitro cultivation and differentiation. Two strains were isolated from HIV+ patients with visceral leishmaniasis (Bibiano and E390M), one strain was isolated from a cutaneous lesion in an immunocompetent patient (HL) and another internal reference strain causative of visceral leishmaniasis (ST) also from an immunocompetent patient was used for comparison. For this objective, we have compared their virulence by in vitro and in vivo infectivity in a murine model of visceral leishmaniasis. RESULTS: Molecular typing unraveled a new k26 sequence attributed to MON-284 zymodeme and allowed the generation of a molecular signature for the identification of each strain. In vitro cultivation enabled the production of promastigotes with comparable growth curves and metacyclogenesis development. The HL strain was the most infective, showing the highest parasite loads in vitro that were corroborated with the in vivo assays, 6 weeks post-infection in BALB/c mice. The two strains isolated from HIV+ patients, both belonging to two different zymodemes, revealed different kinetics of infection. CONCLUSION: Differences in in vitro and in vivo infectivity found in the murine model were then attributed to intrinsic characteristics of each strain. This work is supported by other studies that present the parasite's inherent features as factors for the multiplicity of clinical manifestations and severity of leishmaniasis.
dc.description.sponsorshipThis work was supported by FCT project number PTDC/BIA‒MIC/11866/2011, FEDER Ciência 2010 project number PTDC/SAU‒FCF/101017/2008 and MICINN project number PIM2010‒ENI00627. JC was supported by fellowship from FCT code SFRH/BD/48626/2008 and CS by Contratos de Técnicos de apoyo a la investigación en el SNS code AES-FIS-2011.
dc.language.isoeng
dc.publisherBioMed Central (BMC) 
dc.type.hasVersionVoR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectLeishmania infantum
dc.subjectClinical isolates
dc.subjectVisceral leishmaniasis
dc.subjectMolecular typing
dc.subjectMetacyclogenesis
dc.subjectInfectivity
dc.subjectTropism
dc.titleCharacterization of the biology and infectivity of Leishmania infantum viscerotropic and dermotropic strains isolated from HIV+ and HIV- patients in the murine model of visceral leishmaniasis
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID23622683
dc.format.volume6
dc.format.number1
dc.format.page122
dc.identifier.doi10.1186/1756-3305-6-122
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.description.peerreviewed
dc.identifier.e-issn1756-3305
dc.relation.publisherversionhttps://doi.org/10.1186/1756-3305-6-122
dc.identifier.journalParasites & Vectors
dc.repisalud.centroISCIII::Centro Nacional de Microbiología::Área de Bacteriología, Micología Y Parasitología::Servicio de Parasitología::Unidad de Leishmania
dc.repisalud.institucionISCIII
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional