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dc.contributor.authorIzquierdo-Serrano, Raúl
dc.contributor.authorFernández-Delgado, Irene
dc.contributor.authorMoreno-Gonzalo, Olga 
dc.contributor.authorMartín-Gayo, Enrique
dc.contributor.authorCalzada-Fraile, Diego
dc.contributor.authorRamírez-Huesca, Marta
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorAbián, Joaquín
dc.contributor.authorVicente-Manzanares, Miguel
dc.contributor.authorVeiga, Esteban
dc.contributor.authorVázquez, Jesús
dc.contributor.authorSánchez-Madrid, Francisco
dc.identifier.citationFront Immuno. 2022 Sep 5;13:946358es_ES
dc.description.abstractCommunication through cell-cell contacts and extracellular vesicles (EVs) enables immune cells to coordinate their responses against diverse types of pathogens. The function exerted by EVs in this context depends on the proteins and nucleic acids loaded into EVs, which elicit specific responses involved in the resolution of infection. Several mechanisms control protein and nucleic acid loading into EVs; in this regard, acetylation has been described as a mechanism of cellular retention during protein sorting to exosomes. HDAC6 is a deacetylase involved in the control of cytoskeleton trafficking, organelle polarity and cell migration, defense against Listeria monocytogenes (Lm) infection and other immune related functions. Here, we show that the protein content of dendritic cells (DCs) and their secreted EVs (DEVs) vary during Lm infection, is enriched in proteins related to antiviral functions compared to non-infected cells and depends on HDAC6 expression. Analyses of the post-translational modifications revealed an alteration of the acetylation and ubiquitination profiles upon Lm infection both in DC lysates and DEVs. Functionally, EVs derived from infected DCs upregulate anti-pathogenic genes (e.g. inflammatory cytokines) in recipient immature DCs, which translated into protection from subsequent infection with vaccinia virus. Interestingly, absence of Listeriolysin O in Lm prevents DEVs from inducing this anti-viral state. In summary, these data underscore a new mechanism of communication between bacteria-infected DC during infection as they alert neighboring, uninfected DCs to promote antiviral responses.es_ES
dc.description.sponsorshipThis study was supported by grant PDI-2020-120412RB-I00, PDC2021-121797-I00, BIO2015-67580-P and PGC2018-097019-BI00 from the Spanish Ministry of Economy and Competitiveness (MINECO), grant S2017/BMD-3671-INFLAMUNE-CM from the Comunidad de Madrid, a grant from the Ramón Areces Foundation “Ciencias de la Vida y la Salud” (XIX Concurso-2018), “la Caixa” Banking Foundation (grants HR17-00016 and HR17-00247), BIOIMID (PIE13/041) and PRB3 (IPT17/0019 - ISCIII-SGEFI/ ERDF, ProteoRed) from Instituto de Salud Carlos III, CIBER Cardiovascular (CB16/11/00272), and Fondo de Investigación Sanitaria del Instituto de Salud Carlos III and co-funding by Fondo Europeo de Desarrollo Regional FEDER). IF-D is supported by a Fellowship from the Spanish Ministry of Science, Innovation, and Universities (FPU15/02539). DC-F is supported by a Fellowship from “la Caixa” Foundation (LCF/BQ/DR19/11740010). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX2020- 001041-S). Funding agencies did not intervene in the design of the studies, with no copyright over the study.es_ES
dc.publisherFrontiers Media es_ES
dc.subject.meshExtracellular Vesicles es_ES
dc.subject.meshListeria monocytogenes es_ES
dc.subject.meshListeriosis es_ES
dc.subject.meshNucleic Acids es_ES
dc.subject.meshAntiviral Agents es_ES
dc.subject.meshCytokines es_ES
dc.subject.meshDendritic Cells es_ES
dc.subject.meshHumans es_ES
dc.subject.meshImmunity, Innate es_ES
dc.titleExtracellular vesicles from Listeria monocytogenes-infected dendritic cells alert the innate immune response.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España) es_ES
dc.contributor.funderFundación Ramón Areces es_ES
dc.contributor.funderFundación La Caixa es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderCentro de Investigación Biomédica en Red - CIBERCV (Enfermedades Cardiovasculares) es_ES
dc.contributor.funderFundación ProCNIC es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) es_ES
dc.identifier.journalFrontiers in immunologyes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Inmunobiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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