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dc.contributor.author | Torrente-Rodríguez, Rebeca M | |
dc.contributor.author | Montero-Calle, Ana Maria | |
dc.contributor.author | San Bartolomé, Clara | |
dc.contributor.author | Cano, Olga | |
dc.contributor.author | Vazquez-Alcaraz, Monica | |
dc.contributor.author | Iglesias-Caballero, Maria | |
dc.contributor.author | Corral-Lugo, Andres | |
dc.contributor.author | McConnell, Michael J | |
dc.contributor.author | Pascal, Mariona | |
dc.contributor.author | Mas-Lloret, Vicente | |
dc.contributor.author | Pingarrón, José M | |
dc.contributor.author | Barderas Manchado, Rodrigo | |
dc.contributor.author | Campuzano, Susana | |
dc.date.accessioned | 2022-11-15T12:38:40Z | |
dc.date.available | 2022-11-15T12:38:40Z | |
dc.date.issued | 2022-07-11 | |
dc.identifier.citation | Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202203662. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/15150 | |
dc.description.abstract | The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H2 O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection. | es_ES |
dc.description.sponsorship | The financial support of PID2019-103899RB-I00 (Spanish Ministerio de Ciencia e Innovación) Research Project and the TRANSNANOAVANSENS-CM Program from the Comunidad de Madrid (Grant S2018/NMT-4349) is grate fully acknowledged. R.B. acknowledges the financial support of the PI20CIII/00019 grant from the AES-ISCIII program. R.M.T.-R. acknowledges Talento-Contract from Comunidad de Madrid (2019-T2/IND-15965). A.M.-C. ac knowledges a FPU predoctoral contract supported by the Spanish Ministerio de Educación, Cultura y Deporte. V.M. acknowledges the financial support of the COV20_00679 grant of the COVID-19 Research Call (ISCIII-Spanish Ministry of Science and Innovation). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Electroanalytical Bioplatforms | es_ES |
dc.subject | Immune Response | es_ES |
dc.subject | Omicron | es_ES |
dc.subject | SARS-CoV-2 | es_ES |
dc.subject | Viral Antigens | es_ES |
dc.subject.mesh | Biosensing Techniques | es_ES |
dc.subject.mesh | COVID-19 | es_ES |
dc.subject.mesh | Antibodies, Viral | es_ES |
dc.subject.mesh | COVID-19 Testing | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Immunity | es_ES |
dc.subject.mesh | Immunoglobulin G | es_ES |
dc.subject.mesh | SARS-CoV-2 | es_ES |
dc.subject.mesh | Spike Glycoprotein, Coronavirus | es_ES |
dc.title | Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Attribution-NonCommercial 4.0 Internacional | * |
dc.identifier.pubmedID | 35507573 | es_ES |
dc.format.volume | 61 | es_ES |
dc.format.number | 28 | es_ES |
dc.format.page | e202203662 | es_ES |
dc.identifier.doi | 10.1002/anie.202203662 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Ministerio de Educación, Cultura y Deporte (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1521-3773 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1002/anie.202203662 | es_ES |
dc.identifier.journal | Angewandte Chemie (International ed. in English) | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.centro | ISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC) | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2019-103899RB-I00 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/PI20CIII/00019 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/COV20_00679 | es_ES |