Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/15150
Título
Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors
Autor(es)
Torrente-Rodríguez, Rebeca M | Montero-Calle, Ana Maria ISCIII | San Bartolomé, Clara | Cano, Olga ISCIII | Vazquez-Alcaraz, Monica ISCIII | Iglesias-Caballero, Maria ISCIII | Corral-Lugo, Andres ISCIII | McConnell, Michael J ISCIII | Pascal, Mariona | Mas-Lloret, Vicente ISCIII | Pingarrón, José M | Barderas Manchado, Rodrigo ISCIII | Campuzano, Susana
Fecha de publicación
2022-07-11
Cita
Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202203662.
Idioma
Inglés
Tipo de documento
research article
Resumen
The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H2 O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.
Palabras clave
MESH
Biosensing Techniques | COVID-19 | Antibodies, Viral | COVID-19 Testing | Humans | Immunity | Immunoglobulin G | SARS-CoV-2 | Spike Glycoprotein, Coronavirus
Versión en línea
DOI
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