Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/15150
Title
Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors
Author(s)
Torrente-Rodríguez, Rebeca M | Montero-Calle, Ana Maria ISCIII | San Bartolomé, Clara | Cano, Olga ISCIII | Vazquez-Alcaraz, Monica ISCIII | Iglesias-Caballero, Maria ISCIII | Corral-Lugo, Andres ISCIII | McConnell, Michael J ISCIII | Pascal, Mariona | Mas-Lloret, Vicente ISCIII | Pingarrón, José M | Barderas Manchado, Rodrigo ISCIII | Campuzano, Susana
Date issued
2022-07-11
Citation
Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202203662.
Language
Inglés
Document type
research article
Abstract
The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H2 O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.
Subject
MESH
Biosensing Techniques | COVID-19 | Antibodies, Viral | COVID-19 Testing | Humans | Immunity | Immunoglobulin G | SARS-CoV-2 | Spike Glycoprotein, Coronavirus
Online version
DOI
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