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dc.contributor.authorFebrer-Sendra, Begoña
dc.contributor.authorFernández-Soto, Pedro
dc.contributor.authorCrego-Vicente, Beatriz
dc.contributor.authorGarcía-Bernalt Diego, Juan
dc.contributor.authorTa Tang, Thuy-Huong 
dc.contributor.authorBerzosa, Pedro 
dc.contributor.authorNguema, Rufino
dc.contributor.authorNcogo, Policarpo
dc.contributor.authorRomay-Barja, Maria 
dc.contributor.authorHerrador, Zaida 
dc.contributor.authorBenito, Agustin 
dc.contributor.authorMuro, Antonio
dc.date.accessioned2022-10-18T12:05:41Z
dc.date.available2022-10-18T12:05:41Z
dc.date.issued2022-04-25
dc.identifier.citationDiagnostics (Basel). 2022 Apr 25;12(5):1079.es_ES
dc.identifier.issn2075-4418es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/15065
dc.description.abstractLoiasis, caused by the filarial nematode Loa loa, is endemic in Central and West Africa. Loa loa has been associated with severe adverse reactions in high Loa-infected individuals receiving ivermectin during mass drug administration programs for the control of onchocerciasis and lymphatic filariasis. Diagnosis of loiasis still depends on microscopy in blood samples, but this is not effective for large-scale surveys. New diagnostics methods for loiasis are urgently needed. Previously, we developed a colorimetric high-sensitive and species-specific LAMP for Loa loa DNA detection. Here, we evaluate it in a set of 100 field-collected clinical samples stored as dried blood spots. In addition, Loa loa-LAMP was also evaluated in real-time testing and compared with microscopy and a specific PCR/nested PCR. A simple saponin/Chelex-based method was used to extract DNA. Colorimetric and real-time LAMP assays detected more samples with microscopy-confirmed Loa loa and Loa loa/Mansonella perstans mixed infections than PCR/nested-PCR. Samples with the highest Loa loa microfilariae counts were amplified faster in real-time LAMP assays. Our Loa loa-LAMP could be a promising molecular tool for the easy, rapid and accurate screening of patients for loiasis in endemic areas with low-resource settings. The real-time testing (feasible in a handheld device) could be very useful to rule out high-microfilariae loads in infected patients.es_ES
dc.description.sponsorshipThis research was funded by the Institute of Health Carlos III, ISCIII, Spain (www.isciii.es), grants: RICET RD16/0027/0018 (A.M.), RD16/0027/0000 (A.B.), FCSAI-ISCIII (P.N.) and PI19/01727 (P.F.-S.), European Union co-financing by FEDER (Fondo Europeo de Desarrollo Regional) ‘Una manera de hacer Europa’. We also acknowledge support by the Predoctoral Fellowship Program of Junta de Castilla y León co-financing by Fondo Social Europeo (BDNS (Identif.): 422058 and BDNS (Identif.): 487971), by the ISCIII-Sara Borrell contract CD17CIII/00018 financed by the Institute of Health Carlos III and Predoctoral Fellowship Program of University of Salamanca, and co-financing by Santander Bank.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectLoa loaes_ES
dc.subjectPCRes_ES
dc.subjectColorimetric LAMPes_ES
dc.subjectDried blood spotses_ES
dc.subjectLoiasises_ES
dc.subjectMicroscopyes_ES
dc.subjectMolecular diagnosises_ES
dc.subjectNested-PCRes_ES
dc.subjectReal-time LAMPes_ES
dc.subjectSaponin/Chelexes_ES
dc.titleColorimetric and Real-Time Loop-Mediated Isothermal Amplification (LAMP) for Detection of Loa loa DNA in Human Blood Sampleses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID35626235es_ES
dc.format.volume12es_ES
dc.format.number5es_ES
dc.format.page1079es_ES
dc.identifier.doi10.3390/diagnostics12051079es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderUniversity of Salamanca (España)es_ES
dc.contributor.funderBanco Santander es_ES
dc.contributor.funderJunta de Castilla y León (España) es_ES
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) es_ES
dc.contributor.funderUnión Europea. Fondo Social Europeo (ESF/FSE) es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/diagnostics12051079es_ES
dc.identifier.journalDiagnostics (Basel, Switzerland)es_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/422058es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/487971es_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/MINECO//RD16%2F0027%2F0018/ES/Red de Investigación Colaborativa en Enfermedades Tropicales RICET/ es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/PI19%2F01727/ES/MULTIPLEOX LAMPORTABLE SYSTEM: UNA PLATAFORMA DE DIAGNOSTICO MOLECULAR MULTIPLE BASADO EN TECNOLOGIA LAMP PARA LA IDENTIFICACION ETIOLOGICA DE EOSINOFILIAS IMPORTADAS DE DIFICIL DIAGNOSTICO/ es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/MINECO//RD16%2F0027%2F0001/ES/Red de Investigación Colaborativa en Enfermedades Tropicales RICET/ es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/CD17CIII/00018es_ES


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Atribución 4.0 Internacional
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