Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/14942
903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
Arca de Lafuente, Sonia ISCIII | Casanueva-Benítez, Cristina | Crespo-Bermejo, Celia ISCIII | Lara-Aguilar, Violeta ISCIII | Martín-Carbonero, Luz | de los Santos, Ignacio | Madrid, Ricardo | Briz, Veronica ISCIII
Sci Rep. 2022 Jun 16;12(1):10124.
Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are a useful sampling collecting device for dry blood spot (DBS) storage, especially for low and middle-income countries (LMIC). In this study, we analyzed viral HCV RNA integrity for long-term storage at room temperature compared to − 20 °C using two diferent types of cards for DBS: FTA Classic and 903 Protein Saver cards. For this purpose, DBS were prepared on these cards using blood or plasma samples from HCV infected patients, and samples were analysed by conventional RT-PCR. Our results showed that 903 Protein Saver cards are the best and cheapest alternative for DBS storage at room temperature. In these conditions, we found that HCV RNA integrity lasted for up to 9 months.
Hepatitis C | RNA, Viral | Dried Blood Spot Testing | Hepacivirus | Humans | Sensitivity and Specificity | Specimen Handling | Temperature
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