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dc.contributor.authorHailu, Tadesse
dc.contributor.authorAmor Aramendia, Aranzazu 
dc.contributor.authorNibret, Endalkachew
dc.contributor.authorMunshea, Abaineh
dc.contributor.authorAnegagrie, Melaku 
dc.contributor.authorFlores-Chavez, Maria 
dc.contributor.authorTa Tang, Thuy-Huong 
dc.contributor.authorSaugar, Jose Maria 
dc.contributor.authorBenito, Agustin 
dc.date.accessioned2022-05-24T07:51:16Z
dc.date.available2022-05-24T07:51:16Z
dc.date.issued2022-03-28
dc.identifier.citationBMC Infect Dis. 2022 Mar 28;22(1):297.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14472
dc.description.abstractBackground: Strongyloides stercoralis is an intestinal parasite that can cause chronic infection, hyperinfection and/or a dissemination syndrome in humans. The use of techniques targeting ova fails to detect S. stercoralis, as only larvae of the parasite are excreted in faeces. Due to the absence of "Gold" standard diagnostic method for S. stercoralis, there is a paucity of reported data worldwide. Objective: This study aimed to evaluate the performance of diagnostic methods of S. stercoralis infection by taking the composite reference as a "Gold" standard. Methods: A cross-sectional study was conducted among 844 schoolchildren in Amhara Region, Ethiopia, from April to December 2019. Stool samples were collected and processed with formol-ether concentration technique (FECT), spontaneous tube sedimentation technique (STST), Baermann concentration technique (BCT), agar plate culture (APC) and real-time polymerase chain reaction (RT-PCR). Sensitivity, specificity, positive predictive value, and negative predictive value of each diagnostic method were computed against the composite reference. The agreements of diagnostic methods were evaluated by Kappa value at 95% CI. Results: The composite detection rate of S. stercoralis by the five diagnostic methods was 39.0% (329/844). The detection rate of the parasite from stool samples by FECT, STST, BCT, APC and RT-PCR was 2.0% (17/844), 4.0% (34/844), 10.2% (86/844), 10.9% (92/844) and 28.8% (243/844), respectively. The highest detection rate (37.8%; 319/844) of S. stercoralis was recorded by a combination of BCT, APC, and RT-PCR followed by a combination of STST, BCT, APC and RT-PCR (37.3%; 315/844). The sensitivity of FECT, STST, BCT, APC and RT-PCR against the composite reference was 5.2%, 10.3%, 26.4%, 28.0% and 73.9%, respectively. The diagnostic agreements of RT-PCR, APC, BCT, STST and FECT with the composite reference in detection of S. stercoralis were substantial (0.775), fair (0.321), fair (0.305), slight (0.123), and slight (0.062), respectively. Conclusion: RT-PCR detected the highest number of S. stercoralis infections. A combination of RT-PCR with APC and/or BCT better detected S. stercoralis from stool samples compared to other combinations or single diagnostic methods. Therefore, RT-PCR and combination of RT-PCR with APC and/or BCT diagnostic methods should be advocated for detection of S. stercoralis infection.es_ES
dc.description.sponsorshipThe fund obtained from Bahir Dar University and Mundo Sano Foundation was used for data collection and laboratory detection purpose.es_ES
dc.language.isoenges_ES
dc.publisherBioMed Central (BMC) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAmhara Regiones_ES
dc.subjectDiagnosises_ES
dc.subjectSensitivityes_ES
dc.subjectStrongyloides stercoralises_ES
dc.subjectSpecificityes_ES
dc.subject.meshStrongyloides stercoralis es_ES
dc.subject.meshStrongyloidiasis es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshChild es_ES
dc.subject.meshCross-Sectional Studies es_ES
dc.subject.meshEthiopia es_ES
dc.subject.meshFormaldehyde es_ES
dc.subject.meshHumans es_ES
dc.titleEvaluation of five diagnostic methods for Strongyloides stercoralis infection in Amhara National Regional State, northwest Ethiopiaes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID35346087es_ES
dc.format.volume22es_ES
dc.format.number1es_ES
dc.format.page297es_ES
dc.identifier.doi10.1186/s12879-022-07299-1es_ES
dc.contributor.funderFundación Mundo Sano es_ES
dc.contributor.funderBahir Dar University (Etiopía) es_ES
dc.description.peerreviewedNoes_ES
dc.identifier.e-issn1471-2334es_ES
dc.relation.publisherversionhttps://doi.org/10.1186/s12879-022-07299-1es_ES
dc.identifier.journalBMC infectious diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Medicina Tropicales_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiología
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional