Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/14469
Título
The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
Autor(es)
Bleriot, Ines | Blasco, Lucia | Pacios, Olga | Fernández-García, Laura | Ambroa, Antón | López, María | Ortiz-Cartagena, Concha | Cuenca, Felipe Fernández | Oteo-Iglesias, Jesus ISCIII | Pascual, Álvaro | Martínez-Martínez, Luis | Domingo-Calap, Pilar | Wood, Thomas K | Tomás, María | Wood, Thomas K.
Fecha de publicación
2022-03-16
Cita
Sci Rep. 2022 Mar 16;12(1):4488.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies have demonstrated that TA systems play a key role in phage inhibition. The aim of the present study was to investigate the role of the PemIK (PemK/PemI) type II TA system in phage inhibition by its intrinsic expression in clinical strains of Klebsiella pneumoniae carrying the lncL plasmid, which harbours the carbapenemase OXA-48 and the PemK/PemI TA system. Furthermore, induced expression of the system in an IPTG-inducible plasmid in a reference strain of K. pneumoniae ATCC10031 was also studied. The results showed that induced expression of the whole TA system did not inhibit phage infection, whereas overexpression of the pemK toxin prevented early infection. To investigate the molecular mechanism involved in the PemK toxin-mediated inhibition of phage infection, assays measuring metabolic activity and viability were performed, revealing that overexpression of the PemK toxin led to dormancy of the bacteria. Thus, we demonstrate that the PemK/PemI TA system plays a role in phage infection and that the action of the free toxin induces a dormant state in the cells, resulting in inhibition of phage infections.
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Versión en línea
DOI
Aparece en las colecciones
- Investigación > IIS > INIBIC - Instituto de Investigación Biomédica A Coruña (Galicia)
- Investigación > IIS > IMIBIC - Instituto Maimónides de Investigación Biomédica de Córdoba (Andalucía)
- Investigación > IIS > IBIS - Instituto de Biomedicina de Sevilla (Andalucía)
- Investigación > ISCIII > Centro Nacional de Microbiología (CNM) > ISCIII - Artículos
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