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dc.contributor.authorTrevijano-Contador, Nuria 
dc.contributor.authorRoselletti, Elena
dc.contributor.authorGarcia-Rodas, Rocio 
dc.contributor.authorVecchiarelli, Anna
dc.contributor.authorZaragoza, Oscar 
dc.date.accessioned2022-05-23T10:29:02Z
dc.date.available2022-05-23T10:29:02Z
dc.date.issued2022-02-05
dc.identifier.citationMicroorganisms. 2022 Feb 5;10(2):373.es_ES
dc.identifier.issn2076-2607es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14453
dc.description.abstractCryptococcus neoformans is a pathogenic yeast that can form Titan cells in the lungs, which are fungal cells of abnormally large size. The factors that regulate Titan cell formation in vivo are still unknown, although an increased proportion of these fungal cells of infected mice correlates with induction of Th2-type responses. Here, we focused on the role played by the cytokine IL-17 in the formation of cryptococcal Titan cells using Il17a-/- knockout mice. We found that after 9 days of infection, there was a lower proportion of Titan cells in Il17a-/- mice compared to the fungal cells found in wild-type animals. Dissemination to the brain occurred earlier in Il17a-/- mice, which correlated with the lower proportion of Titan cells in the lungs. Furthermore, knockout-infected mice increased brain size more than WT mice. We also determined the profile of cytokines accumulated in the brain, and we found significant differences between both mouse strains. We found that in Il17a-/-, there was a modest increase in the concentrations of the Th1 cytokine TNF-α. To validate if the increase in this cytokine had any role in cryptococcal morphogenesis, we injected wild-type mice with TNF-α t and observed that fungal cell size was significantly reduced in mice treated with this cytokine. Our results suggest a compensatory production of cytokines in Il17a-/- mice that influences both cryptococcal morphology and dissemination.es_ES
dc.description.sponsorshipThis work was supported by Grant SAF2017-86912-R and PID 2020-114546RB-100 from the Spanish Ministry for Science and Innovation. Roselletti E. is funded by an international collaboration with the company Lesaffre International Sarl. Garcia-Rodas R. is funded by a “Juan de la Cierva” contract from the Spanish Ministry for Economics, Industry, and Competitivity (Reference: IJCI-2015-25683). Trevijano-Contador N. is funded by an “Ayudas de Atracción de Talento Investigador” contract of the Community of Madrid (Reference: 2019-T2/BMD-14926).es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectC57BL76Jes_ES
dc.subjectCryptococcus neoformanses_ES
dc.subjectIL17es_ES
dc.subjectIl17a−/−es_ES
dc.subjectTitan cellses_ES
dc.titleRole of IL-17 in Morphogenesis and Dissemination of Cryptococcus neoformans during Murine Infectiones_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID35208830es_ES
dc.format.volume10es_ES
dc.format.number2es_ES
dc.format.page373es_ES
dc.identifier.doi10.3390/microorganisms10020373es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) es_ES
dc.contributor.funderComunidad de Madrid es_ES
dc.contributor.funderGroupe Lesaffrees_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms10020373es_ES
dc.identifier.journalMicroorganismses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.relation.projectFECYTESTUDIO DE LAS BASES MOLECULARES Y DE LOS FACTORES QUE INDUCEN CAMBIOS MORFOLOGICOS EN CRYPTOCOCCUS NEOFORMANS Y CARACTERIZACION DE NUEVAS ESTRATEGIAS TERAPEUTICAS SAF2017-86912-R AEI 2017es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/MINECO//IJCI-2015-25683/ES/IJCI-2015-25683/ es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2020-114546RB-100es_ES


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