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dc.contributor.authorLorente, Elena 
dc.contributor.authorMarcilla, Miguel
dc.contributor.authorPatricia Garcia de la Sota
dc.contributor.authorQuijada-Freire, Adriana
dc.contributor.authorMir-Gerrero, Carmen 
dc.contributor.authorLópez, Daniel 
dc.date.accessioned2022-05-05T09:02:22Z
dc.date.available2022-05-05T09:02:22Z
dc.date.issued2021-09-29
dc.identifier.citationInt J Mol Sci. 2021 Sep 29;22(19):10503.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14257
dc.description.abstractIdentification of a natural human leukocyte antigen (HLA) ligandome is a key element to understand the cellular immune response. Advanced high throughput mass spectrometry analyses identify a relevant, but not complete, fraction of the many tens of thousands of self-peptides generated by antigen processing in live cells. In infected cells, in addition to this complex HLA ligandome, a minority of peptides from degradation of the few proteins encoded by the viral genome are also bound to HLA class I molecules. In this study, the standard immunopeptidomics strategy was modified to include the classical acid stripping treatment after virus infection to enrich the HLA ligandome in virus ligands. Complexes of HLA-B*27:05-bound peptide pools were isolated from vaccinia virus (VACV)-infected cells treated with acid stripping after virus infection. The HLA class I ligandome was identified using high throughput mass spectrometry analyses, yielding 37 and 51 natural peptides processed and presented untreated and after acid stripping treatment VACV-infected human cells, respectively. Most of these virus ligands were identified in both conditions, but exclusive VACV ligands detected by mass spectrometry detected on acid stripping treatment doubled the number of those identified in the untreated VACV-infected condition. Theoretical binding affinity prediction of the VACV HLA-B*27:05 ligands and acute antiviral T cell response characterization in the HLA transgenic mice model showed no differences between HLA ligands identified under the two conditions: untreated and under acid stripping condition. These findings indicated that acid stripping treatment could be useful to identify HLA class I ligands from virus-infected cells.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministry of Science and Innovation SAF2014-58052 and “Acción Estratégica en Salud” MPY 388/18 to D.L.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectHLA class Ies_ES
dc.subjectImmunoproteomicses_ES
dc.subjectLigandes_ES
dc.subjectPeptidees_ES
dc.subjectViruses_ES
dc.titleAcid Stripping after Infection Improves the Detection of Viral HLA Class I Natural Ligands Identified by Mass Spectrometryes_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID34638844es_ES
dc.format.volume22es_ES
dc.format.number19es_ES
dc.format.page10503es_ES
dc.identifier.doi10.3390/ijms221910503es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1422-0067es_ES
dc.relation.publisherversionhttps://doi.org/10.3390/ijms221910503es_ES
dc.identifier.journalInternational Journal of Molecular Scienceses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/MINECO//SAF2014-58052-R/ES/DISEÑO DE VACUNAS RECOMBINANTES POLIEPITOPICAS PARA GENERAR RESPUESTAS T CD8+ CONTRA VIRUS EMERGENTES/ es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/MPY388/18es_ES


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