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dc.contributor.author | Garcia-Rios, Estefani | |
dc.contributor.author | Gata-de-Benito, Julia | |
dc.contributor.author | Lopez-Siles, Mireia | |
dc.contributor.author | McConnell, Michael J | |
dc.contributor.author | Perez-Romero, Pilar | |
dc.date.accessioned | 2022-05-05T08:42:18Z | |
dc.date.available | 2022-05-05T08:42:18Z | |
dc.date.issued | 2021-09-29 | |
dc.identifier.citation | Int J Mol Sci. 2021 Sep 29;22(19):10558. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/14255 | |
dc.description.abstract | Human cytomegalovirus (HCMV) continues to be a major cause of morbidity in transplant patients and newborns. However, the functions of many of the more than 282 genes encoded in the HCMV genome remain unknown. The development of bacterial artificial chromosome (BAC) technology contributes to the genetic manipulation of several organisms including HCMV. The maintenance of the HCMV BAC in E. coli cells permits the rapid generation of recombinant viral genomes that can be used to produce viral progeny in cell cultures for the study of gene function. We optimized the Lambda-Red Recombination system to construct HCMV gene deletion mutants rapidly in the complete set of tested genes. This method constitutes a useful tool that allows for the quick generation of a high number of gene deletion mutants, allowing for the analysis of the whole genome to improve our understanding of HCMV gene function. This may also facilitate the development of novel vaccines and therapeutics. | es_ES |
dc.description.sponsorship | This study was supported by the Spanish Ministry of Science, Innovation and University, Instituto de Salud Carlos III Grant/Award Numbers: PI17CIII-00014 (MPY110/18); PI20CIII-00009 (MPY303/20); DTS18CIII/00006 (MPY127/19). E.G.-R. is supported by the Sara Borrell Program (CD18CIII/00007), M.L.-S. is supported by the Sara Borrell Program (CD17CIIII/00017), Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | BAC | es_ES |
dc.subject | Lambda-RED system | es_ES |
dc.subject | Cytomegalovirus | es_ES |
dc.subject | Gene-deletion mutants | es_ES |
dc.subject | Recombination | es_ES |
dc.title | Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 34638896 | es_ES |
dc.format.volume | 22 | es_ES |
dc.format.number | 19 | es_ES |
dc.format.page | 10558 | es_ES |
dc.identifier.doi | 10.3390/ijms221910558 | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1422-0067 | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ijms221910558 | es_ES |
dc.identifier.journal | International Journal of Molecular Sciences | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/DTS-ISCIII 2018 Modalidad Proyectos de Desarrollo Tecnológico en Salud Intramurales. (2018)/DTS18CIII/00006 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/PI17CIII-00014 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/PI20CIII-00009 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/MPY303/20 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/MPY127/19 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/MPY110/18 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/CD18CIII/00007 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/CD17CIIII/00017 | es_ES |