Mostrar el registro sencillo del ítem

dc.contributor.authorOlmeda-López, Héctor
dc.contributor.authorCorral-Lugo, Andres 
dc.contributor.authorMcConnell, Michael J 
dc.date.accessioned2022-05-03T11:48:59Z
dc.date.available2022-05-03T11:48:59Z
dc.date.issued2021-10-16
dc.identifier.citationAntibiotics (Basel). 2021;10(10):1259.es_ES
dc.identifier.issn2079-6382es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14244
dc.description.abstractInactivation of the lipooligosaccharide (LOS) biosynthesis genes lpxA, lpxC and lpxD by ISAba insertion elements results in high-level resistance to colistin in A. baumannii. In the present study, we quantify the rate of spontaneous insertional inactivation of LOS biosynthesis genes by ISAba elements in the ATCC 19606-type strain and two multidrug clinical isolates. Using insertional inactivation of lpxC by ISAba11 in the ATCC 19606 strain as a model, we determine the effect of several subinhibitory concentrations of the antibiotics, namely tetracycline, ciprofloxacin, meropenem, kanamycin and rifampicin, as well as the disinfectants ethanol and chlorhexidine on ISAba11 insertion frequencies. Notably, subinhibitory concentrations of tetracycline significantly increased ISAba11 insertion, and rifampicin completely inhibited the emergence of colistin resistance due to ISAba11 inactivation of lpxC. Sequencing of ISAba11 insertion sites within the lpxC gene demonstrated that insertions clustered between nucleotides 382 and 618 (58.3% of unique insertions detected), indicating that this may be a hotspot for ISAba11 insertion. The alignment of insertion sites revealed a semi-conserved AT-rich consensus sequence upstream of the ISAba11 insertion site, suggesting that ISAba11 insertion sites may be sequence-dependent. This study explores previously uncharacterized aspects regarding the acquisition of colistin resistance through insertional activation in LOS biosynthesis genes in A. baumannii.es_ES
dc.description.sponsorshipThis research was supported by grants MPY 380/18 from the Instituto de Salud Carlos III (ISCIII) awarded to M.J.M. A.C.L. is supported by the Atracción de Talento Program of the Community of Madrid.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAcinetobacter baumanniies_ES
dc.subjectISAbaes_ES
dc.subjectAntibiotic resistancees_ES
dc.subjectColistines_ES
dc.subjectInsertion sequencees_ES
dc.titleEffect of Subinhibitory Concentrations of Antibiotics and Disinfectants on ISAba-Mediated Inactivation of Lipooligosaccharide Biosynthesis Genes in Acinetobacter baumanniies_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID34680840es_ES
dc.format.volume10es_ES
dc.format.number10es_ES
dc.format.page1259es_ES
dc.identifier.doi10.3390/antibiotics10101259es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderComunidad de Madrid es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics10101259es_ES
dc.identifier.journalAntibiotics (Basel, Switzerland)es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/MPY380/18es_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail
Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional