Mostrar el registro sencillo del ítem

dc.contributor.authorAcebo, Paloma 
dc.contributor.authorHerranz, Cristina 
dc.contributor.authorEspenberger, Lucas Bernal
dc.contributor.authorGomez-Sanz, Alicia 
dc.contributor.authorTerrón-Orellana, Maria Carmen 
dc.contributor.authorLuque, Daniel 
dc.contributor.authorAmblar, Monica 
dc.date.accessioned2022-05-03T09:39:25Z
dc.date.available2022-05-03T09:39:25Z
dc.date.issued2021-09-05
dc.identifier.citationMicroorganisms. 2021;9(9):1883.es_ES
dc.identifier.issn2076-2607es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14233
dc.description.abstractStreptococcus pneumoniae is a major cause of morbidity and mortality worldwide, and about 30% of the pneumococcal clinical isolates show type I pili-like structures. These long proteinaceous polymers extending from the bacterial surface are encoded by pilus islet 1 and play major roles in adhesion and host colonization. Pili expression is bistable and is controlled by the transcriptional activator RlrA. In this work, we demonstrate that the previously identified small noncoding RNA srn135 also participates in pilus regulation. Our findings show that srn135 is generated upon processing of the 5'-UTR region of rrgA messenger and its deletion prevents the synthesis of RrgA, the main pili adhesin. Moreover, overexpression of srn135 increases the expression of all pili genes and rises the percentage of piliated bacteria within a clonal population. This regulation is mediated by the stabilization of rlrA mRNA since higher levels of srn135 increase its half-life to 165%. Our findings suggest that srn135 has a dual role in pilus expression acting both in cis- (on the RrgA levels) and in trans- (modulating the levels of RlrA) and contributes to the delicate balance between pili expressing and non-expressing bacteria.es_ES
dc.description.sponsorshipThis research was funded by Fondo de Investigación Sanitaria (FIS), grant number PI11/00656, and Ministerio de Economía, Industria y Competitividad, grant number BIO2017-82951-R.” P.A. was the recipient of a contract funded by the FIS project PI11/00656 and A.G.-S. was the recipient of a contract funded by Instituto de Salud Carlos III (CA10/1103).es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPI-1es_ES
dc.subjectPilus-1 typees_ES
dc.subjectS. pneumoniaees_ES
dc.subjectRegulatory RNAes_ES
dc.subjectsRNAes_ES
dc.titleA Small Non-Coding RNA Modulates Expression of Pilus-1 Type in Streptococcus pneumoniaees_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID34576778es_ES
dc.format.volume9es_ES
dc.format.page1883es_ES
dc.identifier.doi10.3390/microorganisms9091883es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms9091883es_ES
dc.identifier.journalMicroorganismses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Instituto de Investigación de Enfermedades Rarases_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BIO2017-82951-Res_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/PI11/00656es_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/CA10/1103es_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional