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dc.contributor.authorMiguel-Oteo, Maria
dc.contributor.authorJiram, Adela I
dc.contributor.authorTa Tang, Thuy-Huong 
dc.contributor.authorLanza, Marta
dc.contributor.authorHisam, Shamilah
dc.contributor.authorRubio Muñoz, Jose Miguel 
dc.date.accessioned2022-03-29T11:33:05Z
dc.date.available2022-03-29T11:33:05Z
dc.date.issued2017-03
dc.identifier.citationAsian Pac J Trop Med. 2017 Mar;10(3):299-304.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/13887
dc.description.abstractObjective: To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of Plasmodium causing human malaria. Methods: In this study the nested multiplex malaria PCR was redesigned, targeting the 18S rRNA gene, to identify the fifth human Plasmodium species, Plasmodium knowlesi, together with the other human Plasmodium (Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale and Plasmodium malariae) by amplified fragment size using only two amplification processes and including an internal reaction control to avoid false negatives. The technique was validated with 91 clinical samples obtained from patients with malaria compatible symptoms. The technique showed high sensitivity (100%) and specificity (96%) when it was compared to the reference method employed for malaria diagnosis in the Instituto de Salud Carlos Ⅲ and a published real-time PCR malaria assay. Conclusions: The technique designed is an economical, sensitive and specific alternative to current diagnosis methods. Furthermore, the method might be tested in knowlesi-malaria endemic areas with a higher number of samples to confirm the quality of the method.es_ES
dc.description.sponsorshipThis work was supported by AESI-ISCⅢ grant number PI14CⅢ/00014.es_ES
dc.language.isoenges_ES
dc.publisherElsevier es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMalariaes_ES
dc.subjectMolecular diagnosises_ES
dc.subjectNested multiplex PCRes_ES
dc.subjectPlasmodium knowlesies_ES
dc.titleNested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesies_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID28442114es_ES
dc.format.volume10es_ES
dc.format.number3es_ES
dc.format.page299-304es_ES
dc.identifier.doi10.1016/j.apjtm.2017.03.014es_ES
dc.contributor.funderInstituto de Salud Carlos III es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2352-4146es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.apjtm.2017.03.014es_ES
dc.identifier.journalAsian Pacific Journal of Tropical Medicinees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/PI14CⅢ/00014es_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional