Mostrar el registro sencillo del ítem
dc.contributor.author | Kartal, Ece | |
dc.contributor.author | Schmidt, Thomas S B | |
dc.contributor.author | Wirbel, Jakob | |
dc.contributor.author | Maistrenko, Oleksandr M | |
dc.contributor.author | Akanni, Wasiu A | |
dc.contributor.author | Alashkar Alhamwe, Bilal | |
dc.contributor.author | Alves, Renato J | |
dc.contributor.author | Carrato, Alfredo | |
dc.contributor.author | Erasmus, Hans-Peter | |
dc.contributor.author | Estudillo, Lidia | |
dc.contributor.author | Finkelmeier, Fabian | |
dc.contributor.author | Fullam, Anthony | |
dc.contributor.author | Glazek, Anna M | |
dc.contributor.author | Gómez-Rubio, Paulina | |
dc.contributor.author | Hercog, Rajna | |
dc.contributor.author | Jung, Ferris | |
dc.contributor.author | Kandels, Stefanie | |
dc.contributor.author | Kersting, Stephan | |
dc.contributor.author | Langheinrich, Melanie | |
dc.contributor.author | Márquez, Mirari | |
dc.contributor.author | Molero, Xavier | |
dc.contributor.author | Orakov, Askarbek | |
dc.contributor.author | Van Rossum, Thea | |
dc.contributor.author | Raul, Torres-Ruiz | |
dc.contributor.author | Telzerow, Anja | |
dc.contributor.author | Zych, Konrad | |
dc.contributor.author | Benes, Vladimir | |
dc.contributor.author | Zeller, Georg | |
dc.contributor.author | Trebicka, Jonel | |
dc.contributor.author | Bork, Peer | |
dc.contributor.author | Malats, Nuria | |
dc.contributor.author | Molina-Montes, Esther | |
dc.contributor.author | Rodriguez Perales, Sandra | |
dc.contributor.author | Real Arribas, Francisco | |
dc.date.accessioned | 2022-03-29T11:00:15Z | |
dc.date.available | 2022-03-29T11:00:15Z | |
dc.date.issued | 2022-03-08 | |
dc.identifier.citation | Gut . 2022 Mar 8;gutjnl-2021-324755. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/13883 | |
dc.description.abstract | Recent evidence suggests a role for the microbiome in pancreatic ductal adenocarcinoma (PDAC) aetiology and progression. To explore the faecal and salivary microbiota as potential diagnostic biomarkers. We applied shotgun metagenomic and 16S rRNA amplicon sequencing to samples from a Spanish case-control study (n=136), including 57 cases, 50 controls, and 29 patients with chronic pancreatitis in the discovery phase, and from a German case-control study (n=76), in the validation phase. Faecal metagenomic classifiers performed much better than saliva-based classifiers and identified patients with PDAC with an accuracy of up to 0.84 area under the receiver operating characteristic curve (AUROC) based on a set of 27 microbial species, with consistent accuracy across early and late disease stages. Performance further improved to up to 0.94 AUROC when we combined our microbiome-based predictions with serum levels of carbohydrate antigen (CA) 19-9, the only current non-invasive, Food and Drug Administration approved, low specificity PDAC diagnostic biomarker. Furthermore, a microbiota-based classification model confined to PDAC-enriched species was highly disease-specific when validated against 25 publicly available metagenomic study populations for various health conditions (n=5792). Both microbiome-based models had a high prediction accuracy on a German validation population (n=76). Several faecal PDAC marker species were detectable in pancreatic tumour and non-tumour tissue using 16S rRNA sequencing and fluorescence in situ hybridisation. Taken together, our results indicate that non-invasive, robust and specific faecal microbiota-based screening for the early detection of PDAC is feasible. | es_ES |
dc.description.sponsorship | We acknowledge funding from EMBL, CNIO, World Cancer Research (#15-0391), the European Research Council (ERC-AdG-669830 MicrobioS), the BMBF-funded Heidelberg CenterCentre for Human Bioinformatics (HD-HuB) within the German Network for Bioinformatics Infrastructure (de.NBI #031A537B), Fondo de Investigaciones Sanitarias (FIS), Instituto de Salud Carlos III-FEDER, Spain (grant numbers PI15/01573, PI18/01347, FIS PI17/02303); Red Tematica de Investigacion Cooperativa en Cancer, Spain (grant numbers RD12/0036/0034, RD12/0036/0050, RD12/0036/0073); III beca Carmen Delgado/Miguel Perez-Mateo de AESPANC-ACANPAN; EU-6FP Integrated Project (#018771-MOLDIAG-PACA); EU-FP7-HEALTH (#259737-CANCERALIA). Funders had no involvement in the study design, patient enrolment, analysis, manuscript writing or reviewing. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BMJ Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | HUMAN GUT MICROBIOME | es_ES |
dc.subject | REAL-TIME PCR | es_ES |
dc.subject | TUMOR MICROBIOME | es_ES |
dc.subject | ORAL MICROBIOTA | es_ES |
dc.subject | DIVERSITY | es_ES |
dc.subject | ASSOCIATION | es_ES |
dc.subject | BIOMARKERS | es_ES |
dc.subject | METAGENOME | es_ES |
dc.title | A faecal microbiota signature with high specificity for pancreatic cancer. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 35260444 | es_ES |
dc.identifier.doi | 10.1136/gutjnl-2021-324755 | es_ES |
dc.contributor.funder | World Cancer Research Fund International | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea | es_ES |
dc.contributor.funder | Federal Ministry of Education & Research (Alemania) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Red Temática de Investigación Cooperativa en Cáncer (RTICC) (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1468-3288 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1136/gutjnl-2021-324755. | es_ES |
dc.identifier.journal | Gut | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Epidemiología Genética y Molecular | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FIS018771-MOLDIAG-PACA | es_ES |
dc.relation.projectID | nfo:eu-repo/grantAgreement/259737-CANCERALIA | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ERC-AdG-669830 MicrobioS | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/PI15/01573 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/PI18/01347 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/FIS PI17/02303 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/RD12/0036/0034 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/RD12/0036/0050 | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/RD12/0036/0073 | es_ES |