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dc.contributor.authorUdayan, Sreeram
dc.contributor.authorButto, Ludovica F
dc.contributor.authorRossini, Valerio
dc.contributor.authorVelmurugan, Janaki
dc.contributor.authorMartinez-Lopez, Maria 
dc.contributor.authorSancho, David 
dc.contributor.authorMelgar, Silvia
dc.contributor.authorO'Toole, Paul W
dc.contributor.authorNally, Ken
dc.identifier.citationSci Rep. 2021; 11(1):5896es_ES
dc.description.abstractThe mechanisms through which cells of the host innate immune system distinguish commensal bacteria from pathogens are currently unclear. Toll-like receptors (TLRs) are a class of pattern recognition receptors (PRRs) expressed by host cells which recognize microbe-associated molecular patterns (MAMPs) common to both commensal and pathogenic bacteria. Of the different TLRs, TLR2/6 recognize bacterial lipopeptides and trigger cytokines responses, especially to Gram-positive and Gram-negative pathogens. We report here that TLR2 is dispensable for triggering macrophage cytokine responses to different strains of the Gram-positive commensal bacterial species Lactobacillus salivarius. The L. salivarius UCC118 strain strongly upregulated expression of the PRRs, Mincle (Clec4e), TLR1 and TLR2 in macrophages while downregulating other TLR pathways. Cytokine responses triggered by L. salivarius UCC118 were predominantly TLR2-independent but MyD88-dependent. However, macrophage cytokine responses triggered by another Gram-positive commensal bacteria, Bifidobacterium breve UCC2003 were predominantly TLR2-dependent. Thus, we report a differential requirement for TLR2-dependency in triggering macrophage cytokine responses to different commensal Gram-positive bacteria. Furthermore, TNF-α responses to the TLR2 ligand FSL-1 and L. salivarius UCC118 were partially Mincle-dependent suggesting that PRR pathways such as Mincle contribute to the recognition of MAMPs on distinct Gram-positive commensal bacteria. Ultimately, integration of signals from these different PRR pathways and other MyD88-dependent pathways may determine immune responses to commensal bacteria at the host-microbe interface.es_ES
dc.description.sponsorshipThis work was supported by grants from Science Foundation Ireland—namely a research centre Grant (SFI-12/RC/2273) to APC Microbiome Ireland.es_ES
dc.publisherNature Publishing Group es_ES
dc.titleMacrophage cytokine responses to commensal Gram-positive Lactobacillus salivarius strains are TLR2-independent and Myd88-dependent.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderScience Foundation Ireland 
dc.identifier.journalScientific reportses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Imagen de la Inflamación Cardiovascular y la Respuesta Inmunees_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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