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dc.contributor.authorMacias, Alvaro 
dc.contributor.authorde la Cruz, Alicia
dc.contributor.authorPeraza, Diego A
dc.contributor.authorBenito-Bueno, Angela de
dc.contributor.authorGonzalez, Teresa
dc.contributor.authorValenzuela, Carmen
dc.date.accessioned2021-08-26T10:53:45Z
dc.date.available2021-08-26T10:53:45Z
dc.date.issued2021-01-29
dc.identifier.citationInt J Mol Sci. 2021; 22(3):1336es_ES
dc.identifier.issn1422-0067es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/13316
dc.description.abstractKV1.5 channel function is modified by different regulatory subunits. KVβ1.3 subunits assemble with KV1.5 channels and induce a fast and incomplete inactivation. Inhibition of PKC abolishes the KVβ1.3-induced fast inactivation, decreases the amplitude of the current KV1.5-KVβ1.3 and modifies their pharmacology likely due to changes in the traffic of KV1.5-KVβ1.3 channels in a PKC-dependent manner. In order to analyze this hypothesis, HEK293 cells were transfected with KV1.5-KVβ1.3 channels, and currents were recorded by whole-cell configuration of the patch-clamp technique. The presence of KV1.5 in the membrane was analyzed by biotinylation techniques, live cell imaging and confocal microscopy approaches. PKC inhibition resulted in a decrease of 33 ± 7% of channels in the cell surface due to reduced recycling to the plasma membrane, as was confirmed by confocal microscopy. Live cell imaging indicated that PKC inhibition almost abolished the recycling of the KV1.5-KVβ1.3 channels, generating an accumulation of channels into the cytoplasm. All these results suggest that the trafficking regulation of KV1.5-KVβ1.3 channels is dependent on phosphorylation by PKC and, therefore, they could represent a clinically relevant issue, mainly in those diseases that exhibit modifications in PKC activity.es_ES
dc.description.sponsorshipThis research was funded by Ministerio de Ciencia e Innovación (MICINN) Spain SAF2016-75021-R and PID2019-104366RB-C21 (to C.V. and T.G.), the Instituto de Salud Carlos III CIBERCVprogram CB/11/00222 (to C.V.), and the Consejo Superior de Investigaciones Científicas grants: PIE201820E104 and 2019AEP148 (to C.V.). The cost of this publication was paid in part by funds fromthe European Fund for Economic and Regional Development (FEDER). A.M. holds a postdoctoral contract at CNIC. A.d.l.C. and D.A.P. held CSIC contracts. A.d.B.-B. holds an MICINN predoctoralcontract (BES-2017-080184).es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshCell Membrane es_ES
dc.subject.meshCytoplasm es_ES
dc.subject.meshHEK293 Cells es_ES
dc.subject.meshHumans es_ES
dc.subject.meshKv1.3 Potassium Channel es_ES
dc.subject.meshKv1.5 Potassium Channel es_ES
dc.subject.meshPhosphorylation es_ES
dc.subject.meshProtein Kinase C es_ES
dc.titleKV1.5-KVβ1.3 Recycling Is PKC-Dependent.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33572906es_ES
dc.format.volume22es_ES
dc.format.number3es_ES
dc.format.page1336es_ES
dc.identifier.doi10.3390/ijms22031336es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/ijms22031336es_ES
dc.identifier.journalInternational journal of molecular scienceses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Arritmias Cardíacases_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genéticaes_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SAF2016-75021-Res_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2019-104366RB-C21es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/CB/11/00222es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PIE201820E104es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/2019AEP148es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/BES-2017-080184es_ES


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