Mostrar el registro sencillo del ítem

dc.contributor.authorGonzalez, Maria Isabel
dc.contributor.authorGonzalez-Arjona, Mario
dc.contributor.authorSantos-Coquillat, Ana
dc.contributor.authorVaquero, Javier
dc.contributor.authorVazquez-Ogando, Elena
dc.contributor.authorMolina-Iracheta, Antonio 
dc.contributor.authorPeinado Selgas, Hector 
dc.contributor.authorDesco, Manuel 
dc.contributor.authorSalinas, Beatriz 
dc.date.accessioned2021-08-26T09:20:40Z
dc.date.available2021-08-26T09:20:40Z
dc.date.issued2021-01-16
dc.identifier.citationBiomedicines. 2021; 9:81es_ES
dc.identifier.issn2227-9059es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/13313
dc.description.abstractThe vertiginous increase in the use of extracellular vesicles and especially exosomes for therapeutic applications highlights the necessity of advanced techniques for gaining a deeper knowledge of their pharmacological properties. Herein, we report a novel chemical approach for the robust attachment of commercial fluorescent dyes to the exosome surface with covalent binding. The applicability of the methodology was tested on milk and cancer cell-derived exosomes (from U87 and B16F10 cancer cells). We demonstrated that fluorescent labeling did not modify the original physicochemical properties of exosomes. We tested this nanoprobe in cell cultures and healthy mice to validate its use for in vitro and in vivo applications. We confirmed that these fluorescently labeled exosomes could be successfully visualized with optical imaging.es_ES
dc.description.sponsorshipThis study was supported by the Comunidad de Madrid, projects: “Y2018/NMT-4949 (NanoLiver-CM)” and “S2017/BMD-3867 (RENIM-CM)”; it was also co-funded by the European Structural and Investment Fund. The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN), and the Pro CNIC Foundation, and it is a Severo Ochoa Center of Excellence (SEV-2015-0505). JV was supported by grants from Instituto de Salud Carlos III (PI18/01833), co-funded by European Regional Development Fund (ERDF) and from Comunidad de Madrid, project “S2017/BMD2737 (ExoHep-CM)”, co-funded by European Structural and Investment Fund. A. Santos-Coquillat is grateful for the financial support from Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III Sara Borrell Fellowship grant CD19/00136.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleCovalently Labeled Fluorescent Exosomes for In Vitro and In Vivo Applications.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33467033es_ES
dc.format.volume9es_ES
dc.format.number1es_ES
dc.format.page81es_ES
dc.identifier.doi10.3390/biomedicines9010081es_ES
dc.contributor.funderComunidad de Madrid (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderFundación ProCNIC 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/biomedicines9010081es_ES
dc.identifier.journalBiomedicineses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Imagen Avanzadaes_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Medicina Comparativaes_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/Y2018/NMT-4949es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/S2017/BMD-3867es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PI18/01833es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/S2017/BMD2737es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/CD19/00136es_ES


Ficheros en el ítem

Acceso Abierto
Thumbnail
Acceso Abierto
Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional