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dc.contributor.authorGomez-Gaviro, Maria Victoria
dc.contributor.authorSanderson, Daniel
dc.contributor.authorRipoll, Jorge
dc.contributor.authorDesco, Manuel 
dc.date.accessioned2021-06-22T08:31:01Z
dc.date.available2021-06-22T08:31:01Z
dc.date.issued2020-08-21
dc.identifier.citationiScience. 2020; 23(8):101432es_ES
dc.identifier.issn2589-0042es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/13172
dc.description.abstractThree-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a pathological condition. Whole-organ optical imaging requires tissue transparency, which can be achieved by using tissue clearing procedures enabling deeper image acquisition and therefore making possible the analysis of large-scale biological tissue samples. Here, we review currently available clearing agents, methods, and their application in imaging of physiological or pathological conditions in different animal and human organs. We also compare different optical tissue clearing methods discussing their advantages and disadvantages and review the use of different 3D imaging techniques for the visualization and image acquisition of cleared tissues. The use of optical tissue clearing resources for large-scale biological tissues 3D imaging paves the way for future applications in translational and clinical research.es_ES
dc.description.sponsorshipThis work was supported by Ministerio de Ciencia, Innovacion y Universidades, ISCIII-FIS grants PI18/00462 co-financed by ERDF, European Union (FEDER) Funds from the European Commission, European Union, ‘‘A way of making Europe’’; the CNIC is supported by theMinisterio de Ciencia, Innovacion y Universidades y the Pro CNIC Foundation, Severo Ochoa Center of Excellence (SEV-2015-0505), CIBER de Salud Mental (CIBERSAM), and COST-action CA16124. J.R. acknowledges funding from EU H2020 FET Open project SENSITIVE, ID 801347, and Ministerio de Ciencia, Innovacion y Universidades Grant FIS2016-77892-Res_ES
dc.language.isoenges_ES
dc.publisherCell Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleBiomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID32805648es_ES
dc.format.volume23es_ES
dc.format.number8es_ES
dc.format.page101432es_ES
dc.identifier.doi10.1016/j.isci.2020.101432es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)
dc.contributor.funderFundación ProCNIC
dc.contributor.funderCentro de Investigación Biomedica en Red - CIBER
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.isci.2020.101432es_ES
dc.identifier.journaliSciencees_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Imagen Avanzadaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI18/00462es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CA16124es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/801347es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.relation.projectFISinfo:eu-repo/grantAgreement/ES/FIS2016-77892-Res_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional