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dc.contributor.author | Bernal-Martinez, Leticia | |
dc.contributor.author | Herrera-Leon, Laura | |
dc.contributor.author | Valero, Clara | |
dc.contributor.author | de la Cruz, Paula | |
dc.contributor.author | Ghimpu, Larisa | |
dc.contributor.author | Mesa-Arango, Ana Cecilia | |
dc.contributor.author | Santoni, Gabriela | |
dc.contributor.author | Goterris, Lidia | |
dc.contributor.author | Millán, Rosario | |
dc.contributor.author | Buitrago, Maria Jose | |
dc.date.accessioned | 2021-05-05T19:18:00Z | |
dc.date.available | 2021-05-05T19:18:00Z | |
dc.date.issued | 2021-04-27 | |
dc.identifier.citation | J Fungi (Basel) . 2021 Apr 27;7(5):336. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/12884 | |
dc.description.abstract | Opportunistic fungal pneumonias (OFP) are the main cause of death in AIDS patients worldwide. Diagnosis of these infections is often late as tuberculosis (TB) is frequently the first suspicion. In addition, diagnostic tools have limitations and are unavailable in disadvantaged regions. To perform the differential diagnosis of the main fungi causing OFP in AIDS patients (Histoplasma capsulatum, Cryptococcus neoformans/C. gattii and Pneumocystis jirovecii) vs. the Mycobacterium tuberculosis complex (MTBC), two new assays were developed: (i) a multiplex real-time PCR (MRT-PCR) and (ii) a simple and cost-effective method based on real-time PCR and the analysis of melting curves after amplification (MC-PCR). Both of the techniques were optimized and standardized "in vitro", showing a suitable reproducibility (CV ranged between 1.84 and 3.81% and 1.41 and 4.83%, respectively), a 100% specificity and detection limits between 20 and 2 fg of genomic DNA per 20 µL of reaction. A validation study was performed by retrospectively using 42 clinical samples from 37 patients with proven fungal infection or TB, and 33 controls. The overall sensitivity for the MRT-PCR assay and the MC-PCR assay was 88% and 90.4%, respectively. Both techniques were fast, sensitive and reproducible, allowing for the detection of these pathogens and the performance of a differential diagnosis. | es_ES |
dc.description.sponsorship | This work was supported by research projects PI14CIII/00045 and PI17CIII/00033 from Spanish Fondo de Investigaciones Sanitarias of the Instituto de Salud Carlos III. L.B-M. has a contract supported by the Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III, cofinanced by the European Development Regional Fund (EDRF) “A Way to Achieve Europe” and the Spanish Network for the Research in Infectious Diseases (REIPI; RD16/CIII/0004/0003). A.C.M-A had a short fellowship from Fundación Carolina (call 2017–2018). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Differential diagnosis | es_ES |
dc.subject | Fungal pneumonia | es_ES |
dc.subject | Tuberculosis | es_ES |
dc.title | Differential Diagnosis of Fungal Pneumonias vs. Tuberculosis in AIDS Patients by Using Two New Molecular Methods. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 33925404 | es_ES |
dc.format.volume | 7 | es_ES |
dc.format.number | 5 | es_ES |
dc.identifier.doi | 10.3390/jof7050336 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.contributor.funder | RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España) | |
dc.contributor.funder | Fundación Carolina | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2309-608X | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/jof7050336 | es_ES |
dc.identifier.journal | Journal of Fungi (Basel, Switzerland) | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI14CIII/00045 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI17CIII/00033 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD16/CIII/0004/0003 | es_ES |
dc.rights.accessRights | open access | es_ES |