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dc.contributor.authorBernal-Martinez, Leticia 
dc.contributor.authorHerrera-Leon, Laura 
dc.contributor.authorValero, Clara 
dc.contributor.authorde la Cruz, Paula
dc.contributor.authorGhimpu, Larisa
dc.contributor.authorMesa-Arango, Ana C 
dc.contributor.authorSantoni, Gabriela
dc.contributor.authorGoterris, Lidia
dc.contributor.authorMillán, Rosario
dc.contributor.authorBuitrago, Maria Jose
dc.identifier.citationJ Fungi (Basel) . 2021 Apr 27;7(5):336.es_ES
dc.description.abstractOpportunistic fungal pneumonias (OFP) are the main cause of death in AIDS patients worldwide. Diagnosis of these infections is often late as tuberculosis (TB) is frequently the first suspicion. In addition, diagnostic tools have limitations and are unavailable in disadvantaged regions. To perform the differential diagnosis of the main fungi causing OFP in AIDS patients (Histoplasma capsulatum, Cryptococcus neoformans/C. gattii and Pneumocystis jirovecii) vs. the Mycobacterium tuberculosis complex (MTBC), two new assays were developed: (i) a multiplex real-time PCR (MRT-PCR) and (ii) a simple and cost-effective method based on real-time PCR and the analysis of melting curves after amplification (MC-PCR). Both of the techniques were optimized and standardized "in vitro", showing a suitable reproducibility (CV ranged between 1.84 and 3.81% and 1.41 and 4.83%, respectively), a 100% specificity and detection limits between 20 and 2 fg of genomic DNA per 20 µL of reaction. A validation study was performed by retrospectively using 42 clinical samples from 37 patients with proven fungal infection or TB, and 33 controls. The overall sensitivity for the MRT-PCR assay and the MC-PCR assay was 88% and 90.4%, respectively. Both techniques were fast, sensitive and reproducible, allowing for the detection of these pathogens and the performance of a differential diagnosis.es_ES
dc.description.sponsorshipThis work was supported by research projects PI14CIII/00045 and PI17CIII/00033 from Spanish Fondo de Investigaciones Sanitarias of the Instituto de Salud Carlos III. L.B-M. has a contract supported by the Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III, cofinanced by the European Development Regional Fund (EDRF) “A Way to Achieve Europe” and the Spanish Network for the Research in Infectious Diseases (REIPI; RD16/CIII/0004/0003). A.C.M-A had a short fellowship from Fundación Carolina (call 2017–2018).es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectDifferential diagnosises_ES
dc.subjectFungal pneumoniaes_ES
dc.titleDifferential Diagnosis of Fungal Pneumonias vs. Tuberculosis in AIDS Patients by Using Two New Molecular Methods.es_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderRed Española de Investigación en Patología Infecciosaes_ES
dc.contributor.funderFundación Carolinaes_ES
dc.identifier.journalJournal of Fungi (Basel, Switzerland)es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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