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dc.contributor.authorSoto, Manuel
dc.contributor.authorRamírez, Laura
dc.contributor.authorSolana, Jose Carlos 
dc.contributor.authorCook, Emma C. L.
dc.contributor.authorHernández-García, Elena
dc.contributor.authorRequena, José María
dc.contributor.authorIborra, Salvador
dc.date.accessioned2021-04-30T11:33:17Z
dc.date.available2021-04-30T11:33:17Z
dc.date.issued2021-02-12
dc.identifier.citationMicroorganisms. 2021;9(2):363.es_ES
dc.identifier.issn2076-2607es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12847
dc.description.abstractLeishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the LiΔHSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate LiΔHSP70-II as a candidate for the development of human vaccines.es_ES
dc.description.sponsorshipThis research was funded by grants from Ministerio de Ciencia e Innovación FISPI11/00095 and FISPI14/00366 (FEDER FUNDING) to M.S. and RYC-2016-19463 and RTI2018-343 to S.I. J.M.R. and M.S. are funded by the Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/008-FEDER). E.H.G. is supported by a FPI grant from the Spanish Ministerio de Ciencia e Innovación. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBALB/c micees_ES
dc.subjectIFN-γes_ES
dc.subjectLeishmania amazonensises_ES
dc.subjectAttenuated parasiteses_ES
dc.subjectLive vaccineses_ES
dc.subjectMurine leishmaniasises_ES
dc.titleInoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33673117es_ES
dc.format.volume9es_ES
dc.format.number2es_ES
dc.identifier.doi10.3390/microorganisms9020363es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderFundación Ramón Areces 
dc.contributor.funderBanco Santander 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms9020363es_ES
dc.identifier.journalMicroorganismses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/FISPI11/00095es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/FISPI14/00366es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2016-19463es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RTI2018-343es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/ISCIII-RETICRD16/0027/008-FEDERes_ES
dc.rights.accessRightsopen accesses_ES


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