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dc.contributor.authorCorsini, Bruno 
dc.contributor.authorAguinagalde, Leire 
dc.contributor.authorRuiz, Susana
dc.contributor.authorDomenech, Mirian 
dc.contributor.authorYuste, Jose Enrique 
dc.date.accessioned2021-04-20T09:04:29Z
dc.date.available2021-04-20T09:04:29Z
dc.date.issued2021-02-23
dc.identifier.citationVaccines (Basel) . 2021 Feb 23;9(2):186.es_ES
dc.identifier.issn2076-393Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12695
dc.description.abstractThe emergence of non-vaccine serotypes of Streptococcus pneumoniae after the use of vaccines based in capsular polysaccharides demonstrates the need of a broader protection vaccine based in protein antigens and widely conserved. In this study, we characterized three important virulence factors of S. pneumoniae namely LytA, LytC, and Pce as vaccine candidates. These proteins are choline-binding proteins that belong to the cell wall hydrolases' family. Immunization of mice with LytA, LytC, or Pce induced high titers of immunoglobulins G (IgGs) of different subclasses, with IgG1, IgG2a, and IgG2b as the predominant immunoglobulins raised. These antibodies activated the classical pathway of the complement system by increasing the recognition of C1q on the surface of pneumococcal strains of different serotypes. Consequently, the key complement component C3 recognized more efficiently these strains in the presence of specific antibodies elicited by these proteins, activating, therefore, the phagocytosis. Finally, a mouse sepsis model of infection was established, confirming that vaccination with these proteins controlled bacterial replication in the bloodstream, increasing the survival rate. Overall, these results demonstrate that LytA, LytC, and Pce can be protein antigens to be contained in a future universal vaccine against S. pneumoniae.es_ES
dc.description.sponsorshipThis research was funded by Ministerio de Economía, Industria y Competitividad (grant number SAF2017-83388). B.C. and L.A. were supported, respectively, by a fellowship from the Brazilian Program Ciencia Sem Fronteiras (CsF) from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and by an FPI fellowship from MINECO.es_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectLytAes_ES
dc.subjectLytCes_ES
dc.subjectPcees_ES
dc.subjectS. pneumoniaees_ES
dc.subjectcell wall hydrolaseses_ES
dc.subjectcomplementes_ES
dc.subjectphagocytosises_ES
dc.subjectvaccine proteines_ES
dc.titleVaccination with LytA, LytC, or Pce of Streptococcus pneumoniae Protects against Sepsis by Inducing IgGs That Activate the Complement System.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33672306es_ES
dc.format.volume9es_ES
dc.format.number2es_ES
dc.identifier.doi10.3390/vaccines9020186es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/vaccines9020186es_ES
dc.identifier.journalVaccineses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2017-83388es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución 4.0 Internacional
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