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dc.contributor.authorOwen, Sophie I
dc.contributor.authorHossain, Faria
dc.contributor.authorGhosh, Prakash
dc.contributor.authorChowdhury, Rajashree
dc.contributor.authorHossain, Md Sakhawat
dc.contributor.authorJewell, Chris
dc.contributor.authorCruz, Israel 
dc.contributor.authorPicado, Albert
dc.contributor.authorMondal, Dinesh
dc.contributor.authorAdams, Emily R
dc.identifier.citationParasit Vectors . 2021 Feb 17;14(1):111.es_ES
dc.description.abstractAsymptomatic Leishmania infections outnumber clinical infections on the Indian subcontinent (ISC), where disease reservoirs are anthroponotic. Diagnostics which detect active asymptomatic infection, which are suitable for monitoring and surveillance, may be of benefit to the visceral leishmaniasis (VL) elimination campaign on the ISC. Quantitative polymerase chain reaction (qPCR), loop-mediated isothermal amplification (LAMP), and the direct agglutination test (DAT) were carried out on blood samples, and the Leishmania antigen ELISA was carried out on urine samples collected from 720 household and neighbouring contacts of 276 VL and post-kala-azar dermal leishmaniasis (PKDL) index cases, with no symptoms or history of VL or PKDL, in endemic regions of Bangladesh between September 2016 and March 2018. Of the 720 contacts of index cases, asymptomatic infection was detected in 69 (9.6%) participants by a combination of qPCR (1.0%), LAMP (2.1%), DAT (3.9%), and Leishmania antigen ELISA (3.3%). Only one (0.1%) participant was detected positive by all four diagnostic tests. Poor agreement between tests was calculated using Cohen's kappa (κ) statistics; however, the Leishmania antigen ELISA and DAT in combination captured all participants as positive by more than one test. We find evidence for a moderately strong association between the index case being a PKDL case (OR 1.94, p = 0.009), specifically macular PKDL (OR 2.12, p = 0.004), and being positive for at least one of the four tests. Leishmania antigen ELISA on urine detects active asymptomatic infection, requires a non-invasive sample, and therefore may be of benefit for monitoring transmission and surveillance in an elimination setting in combination with serology. Development of an antigen detection test in a rapid diagnostic test (RDT) format would be of benefit to the elimination campaign.es_ES
dc.description.sponsorshipFunding was received for this study from the German Federal Ministry of Education and Research (BMBF) through the KfW Entwicklungs bank, MRC-DTP (MR/N013514/1), and Wellcome Seed fund (108080/Z/15/Z).es_ES
dc.publisherBioMed Central (BMC) es_ES
dc.subjectAsymptomatic Leishmania infectiones_ES
dc.subjectLeishmania antigen ELISAes_ES
dc.subjectVisceral leishmaniasises_ES
dc.titleDetection of asymptomatic Leishmania infection in Bangladesh by antibody and antigen diagnostic tools shows an association with post-kala-azar dermal leishmaniasis (PKDL) patients.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFederal Ministry of Education & Research (Alemania) 
dc.contributor.funderWellcome Seed fund
dc.identifier.journalParasites & vectorses_ES
dc.repisalud.centroISCIII::Escuela Nacional de Sanidades_ES
dc.rights.accessRightsopen accesses_ES

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