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dc.contributor.authorMuadica, Aly Salimo 
dc.contributor.authorKöster, Pamela Carolina 
dc.contributor.authorDashti, Alejandro 
dc.contributor.authorBailo-Barroso, Begoña 
dc.contributor.authorHernandez-De-Mingo, Marta 
dc.contributor.authorBalasegaram, Sooria
dc.contributor.authorCarmena, David 
dc.date.accessioned2021-04-09T09:10:11Z
dc.date.available2021-04-09T09:10:11Z
dc.date.issued2021-02-24
dc.identifier.citationPathogens. 2021 Feb 24;10(3):255.es_ES
dc.identifier.issn2076-0817es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12574
dc.description.abstractInfections by the protist enteroparasites Giardia duodenalis, Cryptosporidium spp., and, to a much lesser extent, Blastocystis sp. are common causes of childhood diarrhoea in low-income countries. This molecular epidemiological study assesses the frequency and molecular diversity of these pathogens in faecal samples from asymptomatic schoolchildren (n = 807) and symptomatic children seeking medical attention (n = 286) in Zambézia province, Mozambique. Detection and molecular characterisation of pathogens was conducted by polymerase chain reaction (PCR)-based methods coupled with Sanger sequencing. Giardia duodenalis was the most prevalent enteric parasite found [41.7%, 95% confidence interval (CI): 38.8‒44.7%], followed by Blastocystis sp. (14.1%, 95% CI: 12.1‒16.3%), and Cryptosporidium spp. (1.6%, 95% CI: 0.9‒2.5%). Sequence analyses revealed the presence of assemblages A (7.0%, 3/43) and B (88.4%, 38/43) within G. duodenalis-positive children. Four Cryptosporidium species were detected, including C. hominis (30.8%; 4/13), C. parvum (30.8%, 4/13), C. felis (30.8%, 4/13), and C. viatorum (7.6%, 1/13). Four Blastocystis subtypes were also identified including ST1 (22.7%; 35/154), ST2 (22.7%; 35/154), ST3 (45.5%; 70/154), and ST4 (9.1%; 14/154). Most of the genotyped samples were from asymptomatic children. This is the first report of C. viatorum and Blastocystis ST4 in Mozambique. Molecular data indicate that anthropic and zoonotic transmission (the latter at an unknown rate) are important spread pathways of diarrhoea-causing pathogens in Mozambique.es_ES
dc.description.sponsorshipThis research was funded by the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain), grant number PI16CIII/00024.es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectBlastocystises_ES
dc.subjectCryptosporidiumes_ES
dc.subjectGiardiaes_ES
dc.subjectMozambiquees_ES
dc.subjectPCRes_ES
dc.subjectChildrenes_ES
dc.subjectDiarrhoeaes_ES
dc.subjectEnteric parasiteses_ES
dc.subjectGenotypinges_ES
dc.subjectMolecular epidemiologyes_ES
dc.titleMolecular Diversity of Giardia duodenalis, Cryptosporidium spp., and Blastocystis sp. in Symptomatic and Asymptomatic Schoolchildren in Zambézia Province (Mozambique).es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID33668348es_ES
dc.format.volume10es_ES
dc.format.number3es_ES
dc.identifier.doi10.3390/pathogens10030255es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/pathogens10030255es_ES
dc.identifier.journalPathogens (Basel, Switzerland)es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI16CIII/00024es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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