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dc.contributor.author | Palacios-Ferrer, José Luis | |
dc.contributor.author | García-Ortega, María Belén | |
dc.contributor.author | Gallardo-Gómez, María | |
dc.contributor.author | García, María Ángel | |
dc.contributor.author | Díaz, Caridad | |
dc.contributor.author | Boulaiz, Houria | |
dc.contributor.author | Valdivia, Javier | |
dc.contributor.author | Jurado, José Miguel | |
dc.contributor.author | Almazan-Fernandez, Francisco M | |
dc.contributor.author | Arias-Santiago, Salvador | |
dc.contributor.author | Amezcua, Víctor | |
dc.contributor.author | Peinado, Héctor | |
dc.contributor.author | Vicente, Francisca | |
dc.contributor.author | Pérez Del Palacio, José | |
dc.contributor.author | Marchal, Juan A | |
dc.contributor.author | Peinado Selgas, Hector | |
dc.date.accessioned | 2021-03-31T11:34:10Z | |
dc.date.available | 2021-03-31T11:34:10Z | |
dc.date.issued | 2021-02-15 | |
dc.identifier.citation | Mol Oncol. 2021;15(2):407-428. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/12497 | |
dc.description.abstract | Malignant melanoma (MM) is the most aggressive and life-threatening form of skin cancer. It is characterized by an extraordinary metastasis capacity and chemotherapy resistance, mainly due to melanoma cancer stem cells (CSCs). To date, there are no suitable clinical diagnostic, prognostic or predictive biomarkers for this neoplasia. Therefore, there is an urgent need for new MM biomarkers that enable early diagnosis and effective disease monitoring. Exosomes represent a novel source of biomarkers since they can be easily isolated from different body fluids. In this work, a primary patient-derived MM cell line enriched in CSCs was characterized by assessing the expression of specific markers and their stem-like properties. Exosomes derived from CSCs and serums from patients with MM were characterized, and their metabolomic profile was analysed by high-resolution mass spectrometry (HRMS) following an untargeted approach and applying univariate and multivariate statistical analyses. The aim of this study was to search potential biomarkers for the diagnosis of this disease. Our results showed significant metabolomic differences in exosomes derived from MM CSCs compared with those from differentiated tumour cells and also in serum-derived exosomes from patients with MM compared to those from healthy controls. Interestingly, we identified similarities between structural lipids differentially expressed in CSC-derived exosomes and those derived from patients with MM such as the glycerophosphocholine PC 16:0/0:0. To our knowledge, this is the first metabolomic-based study aimed at characterizing exosomes derived from melanoma CSCs and patients' serum in order to identify potential biomarkers for MM diagnosis. We conclude that metabolomic characterization of CSC-derived exosomes sets an open door to the discovery of clinically useful biomarkers in this neoplasia. | es_ES |
dc.description.sponsorship | The authors would like to thank Dr Jaime Lazuen and Dr Gustavo Ortiz from the C.I.C. (University of Granada) for the excellent technical assistance in the cytometry analyses, Dr Juan Felix Lopez (BIONAND) for his assistance and advice on electron immunogold labelling microscopy characterization and Daniel Franco (Fundacion MEDINA) for his technical assistance with LC-HRMS analyses. JLP-F (Ref. FPU15/03682) and MG-G (Ref. FPU15/02350) acknowledge the MICIU for providing a PhD fellowship (FPU). This research was supported by the Ministerio de Ciencia, Innovacion y Universidades (MICIU, project nos. MAT2015-62644.C2.2.R and RTI2018-101309-BC2, FEDER Funds), by the Instituto de Salud Carlos III (PIE16-00045), by Consejeria de Economia, Conocimiento, Empresas y Universidad de la Junta de Andalucia and European Regional Development Fund (ERDF), ref. SOMM17/6109/UGR (UCE-PP2017-3), and by the Chair 'Doctors Galera-Requena in cancer stem cell research' (CMC-CTS963). MEDINA authors disclosed the receipt of financial support from Fundacion MEDINA, a public-private partnership of Merck Sharp and Dohme de Espana S.A./Universidad de Granada/Junta de Andalucia. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | HEPATOCELULAR CARCINOMA | es_ES |
dc.subject | THERAPEUTIC TARGET | es_ES |
dc.subject | TUMOR | es_ES |
dc.subject | BIOMARKERS | es_ES |
dc.subject | PLASMA | es_ES |
dc.subject | MICROVESICLES | es_ES |
dc.subject | REVEALS | es_ES |
dc.subject | DISEASE | es_ES |
dc.title | Metabolomic profile of cancer stem cell-derived exosomes from patients with malignant melanoma. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 33052601 | es_ES |
dc.format.volume | 15 | es_ES |
dc.format.number | 2 | es_ES |
dc.format.page | 407-428 | es_ES |
dc.identifier.doi | 10.1002/1878-0261.12823 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.contributor.funder | Unión Europea. Comisión Europea | |
dc.contributor.funder | Regional Government of Andalusia (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1878-0261 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1002/1878-0261.12823. | es_ES |
dc.identifier.journal | Molecular oncology | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Microambiente y Metástasis | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/FPU15/03682 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/FPU15/02350 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PIE16-00045 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/MAT2015-62644.C2.2.R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RTI2018-101309-BC2 | es_ES |
dc.rights.accessRights | open access | es_ES |