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dc.contributor.authorOrtega, Felipe
dc.contributor.authorGomez-Villafuertes, Rosa
dc.contributor.authorBenito-León, María
dc.contributor.authorMartínez de la Torre, Margaret
dc.contributor.authorOlivos-Oré, Luis A.
dc.contributor.authorArribas-Blazquez, Marina
dc.contributor.authorGomez-Gaviro, María Victoria
dc.contributor.authorAzcorra, Arturo
dc.contributor.authorDesco, Manuel 
dc.contributor.authorArtalejo, Antonio R.
dc.contributor.authorPuelles, Luis
dc.contributor.authorMiras-Portugal, María Teresa
dc.date.accessioned2021-03-30T12:02:10Z
dc.date.available2021-03-30T12:02:10Z
dc.date.issued2021-01-12
dc.identifier.citationBrain Struct Funct. 2021es_ES
dc.identifier.issn1863-2661
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12480
dc.description.abstractThe purinergic system is one of the oldest cell-to-cell communication mechanisms and exhibits relevant functions in the regulation of the central nervous system (CNS) development. Amongst the components of the purinergic system, the ionotropic P2X7 receptor (P2X7R) stands out as a potential regulator of brain pathology and physiology. Thus, P2X7R is known to regulate crucial aspects of neuronal cell biology, including axonal elongation, path-finding, synapse formation and neuroprotection. Moreover, P2X7R modulates neuroinflammation and is posed as a therapeutic target in inflammatory, oncogenic and degenerative disorders. However, the lack of reliable technical and pharmacological approaches to detect this receptor represents a major hurdle in its study. Here, we took advantage of the P2rx7-EGFP reporter mouse, which expresses enhanced green fluorescence protein (EGFP) immediately downstream of the P2rx7 proximal promoter, to conduct a detailed study of its distribution. We performed a comprehensive analysis of the pattern of P2X7R expression in the brain of E18.5 mouse embryos revealing interesting areas within the CNS. Particularly, strong labelling was found in the septum, as well as along the entire neural roof plate zone of the brain, except chorioidal roof areas, but including specialized circumventricular roof formations, such as the subfornical and subcommissural organs (SFO; SCO). Moreover, our results reveal what seems a novel circumventricular organ, named by us postarcuate organ (PArcO). Furthermore, this study sheds light on the ongoing debate regarding the specific presence of P2X7R in neurons and may be of interest for the elucidation of additional roles of P2X7R in the idiosyncratic histologic development of the CNS and related systemic functions.es_ES
dc.description.sponsorshipThis work was supported by grants “Red de Excelencia Consolider-Ingenio Spanish Ion Channel Initiative” (BFU2015-70067REDC) from the Ministry of Economy and Competitiveness (BFU2014-53654-P), BRADE-Comunidad de Madrid (S2013/ICE-2958), UCM-Santander (PR26/16-18B-3; PR75/18) and Fundación Ramón Areces Grant program (PR2018/16–02). Maria Benito Leon is recipient of a contract from the “Fondo de Garantía Juvenil, Comunidad de Madrid” CAM PEJD-2016/BMD-2572. Felipe Ortega acknowledges support from the Ramon y Cajal Program of the Spanish Ministry of Economy and Competitiveness (MEC: RYC-2013–13290). This work has been also supported by Comunidad de Madrid, project “S2017/BMD-3867 (RENIM-CM)”, co-funded by European Structural and Investment Fund. The CNIC is supported by the Ministerio de Ciencia, Innovación y Universidades and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015–0505). MVG has been supported by Ministerio de Ciencia, Innovación y Universidades, ISCIII-FIS grants PI18/00462 co-financed by ERDF, European Union (FEDER) Funds from the European Commission, European Union, “A way of making Europe”.
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectP2X7 receptores_ES
dc.subjectPurinergic systemes_ES
dc.subjectEmbryonic braines_ES
dc.subjectP2rx7-EGFP mousees_ES
dc.subjectPostarcuate organes_ES
dc.subjectPArcOes_ES
dc.subjectVentricular hypothalamic organes_ES
dc.titleSalient brain entities labelled in P2rx7-EGFP reporter mouse embryos include the septum, roof plate glial specializations and circumventricular ependymal organses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.doi10.1007/s00429-020-02204-5es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderComunidad de Madrid
dc.contributor.funderUniversidad Complutense de Madrid (España)
dc.contributor.funderBanco Santander
dc.contributor.funderFundación Ramón Areces
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España)
dc.contributor.funderFundación ProCNIC
dc.contributor.funderInstituto de Salud Carlos III - ISCIII
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1007/s00429-020-02204-5es_ES
dc.identifier.journalBrain Structure and Functiones_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Imagen Avanzadaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BFU2015-70067REDCes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BFU2014-53654-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/S2013/ICE-2958es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PR26/16-18B-3es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PR2018/16–02es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CAM/PEJD-2016/BMD-2572es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2013–13290es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/S2017/BMD-3867es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015–0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI18/00462es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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