Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/12478
Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
STAR Protoc. 2021; 2(1):100286
Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes.
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