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dc.contributor.authorAbad, Raquel 
dc.contributor.authorGarcía-Amil, Cristina 
dc.contributor.authorNavarro Rivas, Carmen 
dc.contributor.authorMartín, Elena
dc.contributor.authorMartín-Díaz, Ariadna
dc.contributor.authorVazquez-Moreno, Julio Alberto
dc.identifier.citationJ Infect. 2021 Feb 18;S0163-4453(21)00087-6.es_ES
dc.description.abstractStudies of meningococcal genetic population structure, including the potential associations between surface proteins variants and clonal complexes, are important to understand how new protein MenB vaccines might impact in specific scenarios. With the aim to analyze the diversity of Spanish invasive MenB strains, and genetic variability of the fHbp vaccine antigen, all MenB isolates received at National Reference Laboratory (NRL) from 2015 to 2018 were molecularly characterized. 108, 103, 87 and 112 invasive MenB strains isolated during 2015-2018, respectively, were received at NRL. The strains were whole genome sequenced, and porA, fetA, MLST and fHbp variability was analyzed. Potential impact on MenB vaccines coverage was also assessed. A total of 42, 38 and 3 different FHbp subfamily A, B and A/B hybrid peptides, respectively, were found. FHbp subfamily A peptides were harboured by most of the strains (65.9%), being the most prevalent peptide 45 which was associated with genosubtype 22,14 and cc213. FHbp subfamily B peptides were harboured by 32.4% of the strains, and 6 strains harbouring subfamily A/B hybrid peptides were also found. The 64.15% of the strains showed FHbp variants "exact-match" or "cross-reactive" to the FHbp variants included in rLP2086 vaccine according to hSBA assays in the rLP2086 clinical development, and 15.85% showed FHbp peptides defined as predictors of FHbp-coverage for 4CMenB vaccine by gMATS. Due to invasive meningococcal strains temporal variability (eg prevalence of the cc213 increased from 3.6% in 2007 to 33% in 2018) affecting to the presence and distribution of the vaccine antigens, continuous detailed meningococcal surveillance and monitoring of the vaccine antigens is needed to determine the degree and durability of coverage provided by these protein vaccine.es_ES
dc.description.sponsorshipThis study was partially supported by grants PI16CIII/00023 (MPY-1349/16) and PI19CIII/00030 (MPY-507/19) from Instituto de Salud Carlos III, and by Pfizer S.L.U. through an institutional agreement with the Instituto de Salud Carlos III (MVP-1273/16). This publication made use of the Neisseria Multi Locus Sequence Typing website ( neisseria/) sited at the University of Oxford (Jolley et al. Wellcome Open Res 2018, 3:124 [version 1; referees: 2 approved]). The development of this site has been funded by the Wellcome Trust and European Union. This study made use of Genomic and Bioinformatics ISCIII central Units for genomes sequencing and assembly. We thank all hospitals for sending the strains to the National Reference Laboratory for an IMD laboratory surveillance on a National level.es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectMenB strainses_ES
dc.subjectMenB vaccineses_ES
dc.subjectMolecular characterizationes_ES
dc.titleMolecular characterization of invasive serogroup B Neisseria meningitidis isolates from Spain during 2015-2018: Evolution of the vaccine antigen factor H binding protein (FHbp).es_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III
dc.identifier.journalThe Journal of infectiones_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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