Publication: A kinase-deficient NTRK2 splice variant predominates in glioma and amplifies several oncogenic signaling pathways.
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2020-11-01
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Nature Publishing Group
Abstract
Independent scientific achievements have led to the discovery of aberrant splicing patterns in oncogenesis, while more recent advances have uncovered novel gene fusions involving neurotrophic tyrosine receptor kinases (NTRKs) in gliomas. The exploration of NTRK splice variants in normal and neoplastic brain provides an intersection of these two rapidly evolving fields. Tropomyosin receptor kinase B (TrkB), encoded NTRK2, is known for critical roles in neuronal survival, differentiation, molecular properties associated with memory, and exhibits intricate splicing patterns and post-translational modifications. Here, we show a role for a truncated NTRK2 splice variant, TrkB.T1, in human glioma. TrkB.T1 enhances PDGF-driven gliomas in vivo, augments PDGF-induced Akt and STAT3 signaling in vitro, while next generation sequencing broadly implicates TrkB.T1 in the PI3K signaling cascades in a ligand-independent fashion. These TrkB.T1 findings highlight the importance of expanding upon whole gene and gene fusion analyses to include splice variants in basic and translational neuro-oncology research.
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RNA Splicing Animals Brain Brain Neoplasms Carcinogenesis Cells, Cultured Gene Expression Profiling Gene Ontology Glioma High-Throughput Nucleotide Sequencing Humans Membrane Glycoproteins Mice NIH 3T3 Cells Neural Stem Cells Oncogene Proteins, Fusion Oncogenes Phosphatidylinositol 3-Kinases RNA Isoforms Receptor, trkB Signal Transduction
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Bibliographic citation
Nat Commun.2020;11(1):2977