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dc.contributor.authorBarreiro, Olga 
dc.contributor.authorZamai, Moreno 
dc.contributor.authorYanez-Mo, Maria
dc.contributor.authorTejera, Emilio
dc.contributor.authorLopez-Romero, Pedro 
dc.contributor.authorMonk, Peter N
dc.contributor.authorGratton, Enrico
dc.contributor.authorCaiolfa, Valeria R 
dc.contributor.authorSanchez-Madrid, Francisco 
dc.date.accessioned2021-02-17T10:13:26Z
dc.date.available2021-02-17T10:13:26Z
dc.date.issued2008-11-03
dc.identifier.citationJ Cell Biol. 2008; 183(3):527-42es_ES
dc.identifier.issn0021-9525
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11922
dc.description.abstractVCAM-1 and ICAM-1, receptors for leukocyte integrins, are recruited to cell-cell contact sites on the apical membrane of activated endothelial cells. In this study, we show that this recruitment is independent of ligand engagement, actin cytoskeleton anchorage, and heterodimer formation. Instead, VCAM-1 and ICAM-1 are recruited by inclusion within specialized preformed tetraspanin-enriched microdomains, which act as endothelial adhesive platforms (EAPs). Using advanced analytical fluorescence techniques, we have characterized the diffusion properties at the single-molecule level, nanoscale organization, and specific intradomain molecular interactions of EAPs in living primary endothelial cells. This study provides compelling evidence for the existence of EAPs as physical entities at the plasma membrane, distinct from lipid rafts. Scanning electron microscopy of immunogold-labeled samples treated with a specific tetraspanin-blocking peptide identify nanoclustering of VCAM-1 and ICAM-1 within EAPs as a novel mechanism for supramolecular organization that regulates the leukocyte integrin-binding capacity of both endothelial receptors during extravasation.es_ES
dc.language.isoenges_ES
dc.publisherRockefeller University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAntigens, CD es_ES
dc.subject.meshCell Adhesion es_ES
dc.subject.meshCell Membrane es_ES
dc.subject.meshEndothelium, Vascular es_ES
dc.subject.meshHumans es_ES
dc.subject.meshIntegrins es_ES
dc.subject.meshIntercellular Adhesion Molecule-1 es_ES
dc.subject.meshLeukocytes es_ES
dc.subject.meshMembrane Proteins es_ES
dc.subject.meshMicroscopy, Confocal es_ES
dc.subject.meshMicroscopy, Electron, Scanning es_ES
dc.subject.meshReceptors, Cell Surfacees_ES
dc.subject.meshSignal Transduction es_ES
dc.subject.meshTransfection es_ES
dc.subject.meshTumor Necrosis Factor-alpha es_ES
dc.subject.meshUmbilical Veins es_ES
dc.subject.meshVascular Cell Adhesion Molecule-1 es_ES
dc.titleEndothelial adhesion receptors are recruited to adherent leukocytes by inclusion in preformed tetraspanin nanoplatforms.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID18955551es_ES
dc.format.volume183es_ES
dc.format.number3es_ES
dc.format.page527-42es_ES
dc.identifier.doi10.1083/jcb.200805076es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España)es_ES
dc.contributor.funderFundación Lillyes_ES
dc.contributor.funderNational Institutes of Health (United States)es_ES
dc.contributor.funderEuropean Uniones_ES
dc.contributor.funderRed Temática de Investigación Cooperativa en Enfermedades Cardiovasculares (España)es_ES
dc.contributor.funderMinisterio de Sanidad y Consumo (España)es_ES
dc.contributor.funderFundación ProCNICes_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1083/jcb.200805076es_ES
dc.identifier.journalThe Journal of cell biologyes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoriaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2008-02635es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/2005-08435es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/P41-RRO3155es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/LSHG-CT-2003-502935es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD06/0014-0030es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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