Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/11528
Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain.
Epidemiol Infect . 1992 Jun;108(3):555-7.
Ticks recovered from dogs in rural villages around Madrid (Spain) were processed to isolate rickettsiae. One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to Rickettsia conorii, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultured and identified as a member of the spotted fever group rickettsia. Tick eggs provide suitable material for isolation of rickettsia.
Animals | Antibodies, Bacterial | Boutonneuse Fever | Dogs | Ovum | Rickettsia | Rickettsia Infections | Spain | Ticks | Vero Cells
Files in this item