Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/11526
Use of AFLP, plasmid typing and phenotyping in a comparative study to assess genetic diversity of Shigella flexneri strains
Herrera-Leon, Silvia ISCIII | Cabrera, R ISCIII | Ramirez, M M | Usera, Miguel A. ISCIII | Echeita, Aurora ISCIII
Epidemiol Infect. 2002 Dec;129(3):445-50.
Shigella flexneri infections are one of the main causes of acute diarrhoea in Cuba. Twenty strains isolated from sporadic cases in nine different Cuban provinces were characterized. Serotyping, antibiotic-resistance typing, plasmid-typing and AFLP-typing were used to determine their suitability for use in epidemiological studies of S. flexneri. The predominant serotypes were serotype 6 (35%) and serotype 2 (35%). Eleven different plasmid profiles were detected (Diversity Index = 0.92). AFLP-typing discriminated 12 different patterns (DI = 0.95), these patterns were not coincident with plasmid-typing patterns. Both techniques combined distinguished 14 patterns among the 20 studied strains (DI = 0.99). There was no consistent relationship between plasmid-typing and AFLP-typing patterns or antibiotic-resistance typing patterns. Ninety-five percent of S. flexneri strains were multiresistant.
Polymorphism, Genetic | Cuba | DNA Primers | Diarrhea | Dysentery, Bacillary | Humans | Phenotype | Plasmids | Polymerase Chain Reaction | Polymorphism, Restriction Fragment Length | Serotyping | Shigella flexneri
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