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dc.contributor.authorRodriguez, Jose Manuel 
dc.contributor.authorPozo, Fernando
dc.contributor.authordi Domenico, Tomas
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorTress, Michael 
dc.contributor.authorTress, Michael L
dc.contributor.authorTress, Michael L.
dc.identifier.citationPLoS Comput Biol . 2020;16(10):e1008287.es_ES
dc.description.abstractThe role of alternative splicing is one of the great unanswered questions in cellular biology. There is strong evidence for alternative splicing at the transcript level, and transcriptomics experiments show that many splice events are tissue specific. It has been suggested that alternative splicing evolved in order to remodel tissue-specific protein-protein networks. Here we investigated the evidence for tissue-specific splicing among splice isoforms detected in a large-scale proteomics analysis. Although the data supporting alternative splicing is limited at the protein level, clear patterns emerged among the small numbers of alternative splice events that we could detect in the proteomics data. More than a third of these splice events were tissue-specific and most were ancient: over 95% of splice events that were tissue-specific in both proteomics and RNAseq analyses evolved prior to the ancestors of lobe-finned fish, at least 400 million years ago. By way of contrast, three in four alternative exons in the human gene set arose in the primate lineage, so our results cannot be extrapolated to the whole genome. Tissue-specific alternative protein forms in the proteomics analysis were particularly abundant in nervous and muscle tissues and their genes had roles related to the cytoskeleton and either the structure of muscle fibres or cell-cell connections. Our results suggest that this conserved tissue-specific alternative splicing may have played a role in the development of the vertebrate brain and heart.es_ES
dc.description.sponsorshipThis study was supported by the National Institutes of Health ( grant number 2 U41 HG007234 (FP and MLT), the Spanish Ministry of Science, Innovation and Universities ( grants BIO2015-67580-P and PGC2018-097019-B-I00 (JMR and JV), the Carlos III Institute of HealthFondo de Investigacion ( Sanitaria grant PRB3, IPT17/0019 -ISCIII-SGEFI/ERDF, ProteoRed (JMR and JV), the Fundacio MaratoTV3 ( grant 122/C/2015 (JMR and JV) and "la Caixa" Banking Foundation ( project code HR17-00247 (JMR and JV). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscriptes_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.titleAn analysis of tissue-specific alternative splicing at the protein level.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderUnited States of Department of Health & Human Services 
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderFundación La Marató TV3 
dc.identifier.journalPLoS computational biologyes_ES
dc.repisalud.orgCNIOCNIO::Unidades técnicas::Unidad de Bioinformáticaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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