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dc.contributor.authorSotillo-Gallego, Javier 
dc.contributor.authorRobinson, Mark W
dc.contributor.authorKimber, Michael J
dc.contributor.authorCucher, Marcela
dc.contributor.authorAncarola, María Eugenia
dc.contributor.authorNejsum, Peter
dc.contributor.authorMarcilla, Antonio
dc.contributor.authorEichenberger, Ramon M
dc.contributor.authorTritten, Lucienne
dc.identifier.citationInt J Parasitol . 2020 Aug;50(9):635-645es_ES
dc.description.abstractHelminth parasites have a remarkable ability to persist within their mammalian hosts, which is largely due to their secretion of molecules with immunomodulatory properties. Although the soluble components of helminth secretions have been extensively studied, the discovery that helminths release extracellular vesicles (EVs) has added further complexity to the host-parasite interaction. Whilst several studies have begun to characterise the molecules carried by helminth EVs, work aimed at investigating their biological functions has been hindered by a lack of helminth-specific EV markers. To begin to address this, we summarised helminth EV literature to date. With a focus on the protein and microRNA (miRNA) cargo, we aimed to detect similarities and differences across those major groups of helminths for which data are available; namely nematodes, trematodes and cestodes. Pfam analysis revealed that although there is no universal EV marker for all helminth species, the EF-hand protein family was present in all EV datasets from cestodes and trematodes, and could serve as a platyhelminth EV biomarker. In contrast, M13 metallopeptidases and actin may have potential as markers for nematode EVs. As with proteins, many miRNA families appeared to be species-, stage-, or dataset-specific. Two miRNA families were common to nematode EVs (mir-10 and let-7); the miRNA cargo of EVs secreted by clade I species appeared somewhat different from species from other clades. Five miRNA families (mir-71, mir-10, mir-190, let-7 and mir-2) were shared by all trematode species examined. Our analysis has identified novel markers that may be used in studies aimed at characterising helminth EVs and interrogating their function at the host-parasite interface. In addition, we discuss the heterogeneity of methods used for helminth EV isolation and emphasise the need for a standardised approach in reporting on helminth EV data.es_ES
dc.description.sponsorshipJS is a Miguel Servet Fellow funded by Instituto de Salud Carlos III, Spain (CP17III/00002). AM is supported by the Conselleria d’Educació, Cultura I Esports, Generalitat Valenciana, Valencia, Spain (PROMETEO/2020/071) and by the Agencia Estatal de Investigación, Ministerio de Ciencia e Innovación (PID2019-105713GB-I00/AEI/10.13039/501100011033). MR is supported by a grant (BB/L019612/1) from the Biotechnology and Biological Sciences Research Council, UK (BBSRC). MK is supported by an R21 award (AI117204) from the National Institute of Allergy and Infectious Diseases, USA ( MC and MEA are supported by Perez Guerrero Trust Fund, ANPCyT 2017N°2062 and National Scientific and Technical Research Council (CONICET), Argentina. PN is supported by the Independent Research Fund Denmark (DFF-6111-00521). LT and RME are supported by the Swiss National Science Foundation (PZ00P3_168080 and PZ00P3_185865). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherElsevier es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshExtracellular Vesicles es_ES
dc.subject.meshParasites es_ES
dc.titleThe protein and microRNA cargo of extracellular vesicles from parasitic helminths - current status and research priorities.es_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderGeneralitat Valenciana (España)
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderBiotechnology and Biological Sciences Research Council (Reino Unido)
dc.contributor.funderNIH - National Institute of Allergy and Infectious Diseases (NIAID) (Estados Unidos)
dc.contributor.funderFondo Fiduciario Pérez Guerrero
dc.contributor.funderConsejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
dc.identifier.journalInternational journal for parasitologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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