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dc.contributor.authorPeralta, Marina 
dc.contributor.authorOrtiz Lopez, Laia
dc.contributor.authorJerabkova, Katerina
dc.contributor.authorLucchesi, Tommaso
dc.contributor.authorVitre, Benjamin
dc.contributor.authorHan, Dong
dc.contributor.authorGuillemot, Laurent
dc.contributor.authorDingare, Chaitanya
dc.contributor.authorSumara, Izabela
dc.contributor.authorMercader, Nadia 
dc.contributor.authorLecaudey, Virginie
dc.contributor.authorDelaval, Benedicte
dc.contributor.authorMeilhac, Sigolène M
dc.contributor.authorVermot, Julien
dc.identifier.citationCell Rep. 2020; 32(3):107932es_ES
dc.description.abstractCilia and the intraflagellar transport (IFT) proteins involved in ciliogenesis are associated with congenital heart diseases (CHDs). However, the molecular links between cilia, IFT proteins, and cardiogenesis are yet to be established. Using a combination of biochemistry, genetics, and live-imaging methods, we show that IFT complex B proteins (Ift88, Ift54, and Ift20) modulate the Hippo pathway effector YAP1 in zebrafish and mouse. We demonstrate that this interaction is key to restrict the formation of the proepicardium and the myocardium. In cellulo experiments suggest that IFT88 and IFT20 interact with YAP1 in the cytoplasm and functionally modulate its activity, identifying a molecular link between cilia-related proteins and the Hippo pathway. Taken together, our results highlight a noncanonical role for IFT complex B proteins during cardiogenesis and shed light on a mechanism of action for ciliary proteins in YAP1 regulation.es_ES
dc.description.sponsorshipThis project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant Agreement No. 708312 (M.P.) and from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme: GA No. 682938 (J.V.). This work was supported by FRM (DEQ20140329553), by ANR (ANR-15-CE13-0015–liveheart, ANR- SNF310030E-164245-forcinregeneration), and by the Grant ANR-10-LABX-0030-INRT, a French State fund managed by the Agence Nationale de la Recherche under the frame program Investissements d’Avenir labeled ANR-10-IDEX-0002-02. B.D.’s team was supported by ANR-12-CHEX-005 and CNRS. S.M.M.’s team was supported by core funding from the Institut Imagine, Institut Pasteur, Inserm, Universite´ Paris Descartes, and a grant from the AFM-Te´ le´ thon Trampoline 18727). T.L. was funded by the ED515 (1691/2014). L.O.L. is supported by the European Commission (H2020-MSCA-ITN-2016 European Industrial Doctorate 4DHeart 722427).es_ES
dc.publisherCell Press es_ES
dc.titleIntraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderUnión Europea 
dc.contributor.funderEuropean Research Council 
dc.contributor.funderAgence Nationale de la Recherche (Francia) 
dc.contributor.funderInstitut Pasteur 
dc.contributor.funderInstitut Imagine 
dc.identifier.journalCell reportses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Desarrollo del Epicardio y su Papel en la Regeneraciónes_ES
dc.rights.accessRightsopen accesses_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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