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dc.contributor.authorLogan, Jayden
dc.contributor.authorPearson, Mark S
dc.contributor.authorManda, Srikanth S
dc.contributor.authorChoi, Young-Jun
dc.contributor.authorField, Matthew
dc.contributor.authorEichenberger, Ramon M
dc.contributor.authorMulvenna, Jason
dc.contributor.authorNagaraj, Shivashankar H
dc.contributor.authorFujiwara, Ricardo T
dc.contributor.authorGazzinelli-Guimaraes, Pedro
dc.contributor.authorBueno, Lilian
dc.contributor.authorMati, Vitor
dc.contributor.authorBethony, Jeffrey M
dc.contributor.authorMitreva, Makedonka
dc.contributor.authorLoukas, Alex
dc.contributor.authorSotillo, Javier 
dc.date.accessioned2020-08-10T08:34:30Z
dc.date.available2020-08-10T08:34:30Z
dc.date.issued2020
dc.identifier.citationPLoS Negl Trop Dis . 2020 May 26;14(5):e0008237.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10880
dc.description.abstractThe human hookworm Necator americanus infects more than 400 million people worldwide, contributing substantially to the poverty in these regions. Adult stage N. americanus live in the small intestine of the human host where they inject excretory/secretory (ES) products into the mucosa. ES products have been characterized at the proteome level for a number of animal hookworm species, but until now, the difficulty in obtaining sufficient live N. americanus has been an obstacle in characterizing the secretome of this important human pathogen. Herein we describe the ES proteome of N. americanus and utilize this information along with RNA Seq data to conduct the first proteogenomic analysis of a parasitic helminth, significantly improving the available genome and thereby generating a robust description of the parasite secretome. The genome annotation resulted in a revised prediction of 3,425 fewer genes than initially reported, accompanied by a significant increase in the number of exons and introns, total gene length and the percentage of the genome covered by genes. Almost 200 ES proteins were identified by LC-MS/MS with SCP/TAPS proteins, 'hypothetical' proteins and proteases among the most abundant families. These proteins were compared to commonly used model species of human parasitic infections, including Ancylostoma caninum, Nippostrongylus brasiliensis and Heligmosomoides polygyrus. SCP/TAPS proteins are immunogenic in nematode infections, so we expressed four of those identified in this study in recombinant form and showed that they are all recognized to varying degrees by serum antibodies from hookworm-infected subjects from a disease-endemic area of Brazil. Our findings provide valuable information on important families of proteins with both known and unknown functions that could be instrumental in host-parasite interactions, including protein families that might be key for parasite survival in the onslaught of robust immune responses, as well as vaccine and diagnostic targets.es_ES
dc.description.sponsorshipThis work was supported by a program grant from the National Health and Medical Research Council (NHMRC) [program grant number 1037304] and a Senior Principal Research Fellowship from NHMRC to AL (1117504). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Work performed at Washington University School of Medicine was supported by NIH-NIAID grant AI081803 to MM. JL was supported by an Australian Postgraduate Award and by the Australian Institute of Tropical Health and Medicine at James Cook University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.National Health and Medical Research Council (Australia)National Health and Medical Research Council (Australia)es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshProteome es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshGene Expression Regulation es_ES
dc.subject.meshGene Regulatory Networks es_ES
dc.subject.meshGenome, Helminth es_ES
dc.subject.meshHelminth Proteins es_ES
dc.subject.meshNecator americanus es_ES
dc.subject.meshPhylogeny es_ES
dc.titleComprehensive analysis of the secreted proteome of adult Necator americanus hookworms.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32453752es_ES
dc.format.volume14es_ES
dc.format.number5es_ES
dc.format.pagee0008237es_ES
dc.identifier.doi10.1371/journal.pntd.0008237es_ES
dc.contributor.funderNational Health and Medical Research Council (Australia) 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1935-2735es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0008237es_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/1037304es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/1117504es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional