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dc.contributor.authorMekonnen, Gebeyaw G
dc.contributor.authorTedla, Bemnet A
dc.contributor.authorPickering, Darren
dc.contributor.authorBecker, Luke
dc.contributor.authorWang, Lei
dc.contributor.authorZhan, Bin
dc.contributor.authorBottazzi, Maria Elena
dc.contributor.authorLoukas, Alex
dc.contributor.authorPearson, Mark S
dc.contributor.authorSotillo, Javier
dc.identifier.citationVaccines (Basel). 2020 Jul 24;8(3):E416.es_ES
dc.description.abstractHelminth parasites release extracellular vesicles which interact with the surrounding host tissues, mediating host-parasite communication and other fundamental processes of parasitism. As such, vesicle proteins present attractive targets for the development of novel intervention strategies to control these parasites and the diseases they cause. Herein, we describe the first proteomic analysis by LC-MS/MS of two types of extracellular vesicles (exosome-like, 120 k pellet vesicles and microvesicle-like, 15 k pellet vesicles) from adult Schistosoma haematobium worms. A total of 57 and 330 proteins were identified in the 120 k pellet vesicles and larger 15 k pellet vesicles, respectively, and some of the most abundant molecules included homologues of known helminth vaccine and diagnostic candidates such as Sm-TSP2, Sm23, glutathione S-transferase, saponins and aminopeptidases. Tetraspanins were highly represented in the analysis and found in both vesicle types. Vaccination of mice with recombinant versions of three of these tetraspanins induced protection in a heterologous challenge (S. mansoni) model of infection, resulting in significant reductions (averaged across two independent trials) in liver (47%, 38% and 41%) and intestinal (47%, 45% and 41%) egg burdens. These findings offer insight into the mechanisms by which anti-tetraspanin antibodies confer protection and highlight the potential that extracellular vesicle surface proteins offer as anti-helminth vaccines.es_ES
dc.description.sponsorshipThis work was funded by the NHMRC program grant APP1037304. A.L. was funded by an NHMRC Senior Principal Research Fellowship (APP1117504). B.A.T. was funded by a James Cook University Postgraduate Scholarship. G.G.M. was funded by an AITHM Postgraduate Scholarship.es_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.subject.meshExtracellular Vesicles es_ES
dc.subject.meshSchistosomiasis es_ES
dc.subject.meshTetraspanins es_ES
dc.subject.meshVaccines es_ES
dc.titleSchistosoma haematobium Extracellular Vesicle Proteins Confer Protection in a Heterologous Model of Schistosomiasises_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderNational Health and Medical Research Council (Australia) 
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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