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dc.contributor.authorCarrasco, Gema 
dc.contributor.authorMonzon-Fernandez, Sara 
dc.contributor.authorSan Segundo, Maria 
dc.contributor.authorGarcía, Enrique
dc.contributor.authorGarrido, Noelia 
dc.contributor.authorMedina-Pascual, Maria Jose 
dc.contributor.authorVillalon-Panzano, Pilar 
dc.contributor.authorRamírez, Ana
dc.contributor.authorJimenez Sancho, Maria Pilar 
dc.contributor.authorCuesta de la Plaza, Isabel 
dc.contributor.authorValdezate, Sylvia 
dc.date.accessioned2020-08-10T08:31:05Z
dc.date.available2020-08-10T08:31:05Z
dc.date.issued2020-06-15
dc.identifier.citationMicroorganisms. 2020 Jun 15;8(6):900.es_ES
dc.identifier.issn2076-2607es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10870
dc.description.abstractNocardia species, one of the most predominant Actinobacteria of the soil microbiota, cause infection in humans following traumatic inoculation or inhalation. The identification, typing, phylogenetic relationship and antimicrobial susceptibilities of 38 soil Nocardia strains from Lara State, Venezuela, were studied by 16S rRNA and gyrB (subunit B of topoisomerase II) genes, multilocus sequence analysis (MLSA), whole-genome sequencing (WGS), and microdilution. The results were compared with those for human strains. Just seven Nocardia species with one or two strains each, except for Nocardia cyriacigeorgica with 29, were identified. MLSA confirmed the species assignments made by 16S rRNA and gyrB analyses (89.5% and 71.0% respectively), and grouped each soil strain with its corresponding reference and clinical strains, except for 19 N. cyriacigeorgica strains found at five locations which grouped into a soil-only cluster. The soil strains of N. cyriacigeorgica showed fewer gyrB haplotypes than the examined human strains (13 vs. 17) but did show a larger number of gyrB SNPs (212 vs. 77). Their susceptibilities to antimicrobials were similar except for beta-lactams, fluoroquinolones, minocycline, and clarithromycin, with the soil strains more susceptible to the first three (p ≤ 0.05). WGS was performed on four strains belonging to the soil-only cluster and on two outside it, and the results compared with public N. cyriacigeorgica genomes. The average nucleotide/amino acid identity, in silico genome-to-genome hybridization similarity, and the difference in the genomic GC content, suggest that some strains of the soil-only cluster may belong to a novel subspecies or even a new species (proposed name Nocardia venezuelensis).es_ES
dc.description.sponsorshipThis work was funded by a grant to N.G. from the Instituto de Salud Carlos III (MPY 1278/15). A part of this manuscript forms the M.S project undertaken at the Faculty of Science, Universidad Autónoma de Madrid (Spain).es_ES
dc.language.isoenges_ES
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshMLSAes_ES
dc.subject.meshNocardia cyriacigeorgicaes_ES
dc.subject.meshNocardia sppes_ES
dc.subject.meshWGSes_ES
dc.subject.meshAntimicrobial resistancees_ES
dc.subject.meshgyrBes_ES
dc.subject.meshNocardiosises_ES
dc.subject.meshPotential new species/subspecies N. venezuelensises_ES
dc.subject.meshSoiles_ES
dc.titleMolecular Characterization and Antimicrobial Susceptibilities of Nocardia Species Isolated from the Soil; A Comparison with Species Isolated from Humanses_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32549367es_ES
dc.format.volume8es_ES
dc.format.number6es_ES
dc.identifier.doi10.3390/microorganisms8060900es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms8060900es_ES
dc.identifier.journalMicroorganismses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/MPY 1278/15es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional