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dc.contributor.author | López, Esther | |
dc.contributor.author | Marinaro, Federica | |
dc.contributor.author | de Pedro, María de Los Ángeles | |
dc.contributor.author | Sánchez-Margallo, Francisco Miguel | |
dc.contributor.author | Gomez-Serrano, Maria | |
dc.contributor.author | Ponath, Viviane | |
dc.contributor.author | Pogge von Strandmann, Elke | |
dc.contributor.author | Jorge, Inmaculada | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | Fernández-Pereira, Luis Miguel | |
dc.contributor.author | Crisóstomo, Verónica | |
dc.contributor.author | Alvarez, Veronica | |
dc.contributor.author | Casado, Javier G | |
dc.date.accessioned | 2020-07-08T10:08:00Z | |
dc.date.available | 2020-07-08T10:08:00Z | |
dc.date.issued | 2020-06 | |
dc.identifier.citation | Front Cell Dev Biol. 2020; 8:321 | es_ES |
dc.identifier.issn | 2296-634X | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/10712 | |
dc.description.abstract | Experimental data demonstrated that the regenerative potential and immunomodulatory capacity of cardiosphere-derived cells (CDCs) is mediated by paracrine mechanisms. In this process, extracellular vesicles derived from CDCs (EV-CDCs) are key mediators of their therapeutic effect. Considering the future applicability of these vesicles in human diseases, an accurate preclinical-to-clinical translation is needed, as well as an exhaustive molecular characterization of animal-derived therapeutic products. Based on that, the main goal of this study was to perform a comprehensive characterization of proteins and miRNAs in extracellular vesicles from porcine CDCs as a clinically relevant animal model. The analysis was performed by identification and quantification of proteins and miRNA expression profiles. Our results revealed the presence of clusters of immune-related and cardiac-related molecular biomarkers in EV-CDCs. Additionally, considering that priming stem cells with inflammatory stimuli may increase the therapeutic potential of released vesicles, here we studied the dynamic changes that occur in the extracellular vesicles from IFNγ-primed CDCs. These analyses detected statistically significant changes in several miRNAs and proteins. Notably, the increase in interleukin 6 (IL6) protein, as well as the increase in mir-125b (that targets IL6 receptor) was especially relevant. These results suggest a potential involvement of EV-CDCs in the regulation of the IL6/IL6R axis, with implications in inflammatory-mediated diseases. | es_ES |
dc.description.sponsorship | For this study, EL received a funding from the National Institute of Health Carlos III (ISCIII) through the “Sara Borrell” grant (CD19/00048); FM received a MAFRESA S.L. grant (promoted by Jesús Usón Gargallo); a “PFIS” contract (FI19/00041), 2019 Call Strategic Action in Health 2019 (Acción Estratégica en Salud 2017-2020) to MP; Grant “CB16/11/00494” from CIBER-CV (CB16/11/00494) and Ayuda Grupos de Investigación de Extremadura (GR18199) from Consejería de Economía, Ciencia y Agenda Digital (cofunded by European Regional Development Fund – ERDF) to FS-M; a Postgraduate Scholarship for Foreign Studies (Fundación Ramón Areces, XXXI Edition) to MG-S; grants of the Deutsche Forschungsgemeinschaft (KFO325, 1408/14-1, and GRK2573/1) to EP; JV received funding from CIBER-CV (grant “CB16/11/00277”); Spanish Ministry of Science, Innovation and Universities (grants “BIO2015-67580-P” and “PGC2018-097019-B-I00”), ISCIII – Fondo de Investigación Sanitaria (PRB3 grant “IPT17/0019-ISCIII-SGEFI/ERDF”, ProteoRed), Fundació Marató TV3 (grant “122/C/2015”), and “la Caixa” Banking Foundation (project “HR17-00247”); JC received funding by ISCIII through a “Miguel Servet I” grant “MS17/00021” [co-funded by ERDF/European Social Fund (ESF) “A way to make Europe”/“Investing in your future”], the projects “CP17/00021” and “PI18/0911” (co-funded by ERDF/ESF), and by Junta de Extremadura through a “IB16168” grant (co-funded by ERDF/ESF). CNIC is supported by Instituto de Salud Carlos III (ISCIII), Ministerio de Ciencia, Innovación y Universidades (MCNU) and the Pro CNIC Foundation, and it is a Severo Ochoa Center of Excellence (SEV-2015-0505). Cell culture and in vitro studies were performed at the ICTS Nanbiosis (Unit 14, Stem Cell Therapy). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Frontiers Media | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | The Immunomodulatory Signature of Extracellular Vesicles From Cardiosphere-Derived Cells: A Proteomic and miRNA Profiling. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 32582685 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.page | 321 | es_ES |
dc.identifier.doi | 10.3389/fcell.2020.00321 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.contributor.funder | Centro de Investigación Biomedica en Red - CIBER | |
dc.contributor.funder | Fundación La Marató TV3 | |
dc.contributor.funder | Fundación La Caixa | |
dc.contributor.funder | Fundación ProCNIC | |
dc.contributor.funder | Government of Extremadura (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fcell.2020.00321 | es_ES |
dc.identifier.journal | Frontiers in cell and developmental biology | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Proteómica cardiovascular | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SEV-2015-0505 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/CD19/00048 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/FI19/00041 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/CB16/11/00494 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/CB16/11/00277 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/MS17/00021 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BIO2015-67580-P | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PGC2018-097019-B-I00 | es_ES |
dc.rights.accessRights | open access | es_ES |