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dc.contributor.authorLópez, Esther
dc.contributor.authorMarinaro, Federica
dc.contributor.authorde Pedro, María de Los Ángeles
dc.contributor.authorSánchez-Margallo, Francisco Miguel
dc.contributor.authorGomez-Serrano, Maria 
dc.contributor.authorPonath, Viviane
dc.contributor.authorPogge von Strandmann, Elke
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorFernández-Pereira, Luis Miguel
dc.contributor.authorCrisóstomo, Verónica
dc.contributor.authorAlvarez, Veronica
dc.contributor.authorCasado, Javier G
dc.date.accessioned2020-07-08T10:08:00Z
dc.date.available2020-07-08T10:08:00Z
dc.date.issued2020-06
dc.identifier.citationFront Cell Dev Biol. 2020; 8:321es_ES
dc.identifier.issn2296-634Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10712
dc.description.abstractExperimental data demonstrated that the regenerative potential and immunomodulatory capacity of cardiosphere-derived cells (CDCs) is mediated by paracrine mechanisms. In this process, extracellular vesicles derived from CDCs (EV-CDCs) are key mediators of their therapeutic effect. Considering the future applicability of these vesicles in human diseases, an accurate preclinical-to-clinical translation is needed, as well as an exhaustive molecular characterization of animal-derived therapeutic products. Based on that, the main goal of this study was to perform a comprehensive characterization of proteins and miRNAs in extracellular vesicles from porcine CDCs as a clinically relevant animal model. The analysis was performed by identification and quantification of proteins and miRNA expression profiles. Our results revealed the presence of clusters of immune-related and cardiac-related molecular biomarkers in EV-CDCs. Additionally, considering that priming stem cells with inflammatory stimuli may increase the therapeutic potential of released vesicles, here we studied the dynamic changes that occur in the extracellular vesicles from IFNγ-primed CDCs. These analyses detected statistically significant changes in several miRNAs and proteins. Notably, the increase in interleukin 6 (IL6) protein, as well as the increase in mir-125b (that targets IL6 receptor) was especially relevant. These results suggest a potential involvement of EV-CDCs in the regulation of the IL6/IL6R axis, with implications in inflammatory-mediated diseases.es_ES
dc.description.sponsorshipFor this study, EL received a funding from the National Institute of Health Carlos III (ISCIII) through the “Sara Borrell” grant (CD19/00048); FM received a MAFRESA S.L. grant (promoted by Jesús Usón Gargallo); a “PFIS” contract (FI19/00041), 2019 Call Strategic Action in Health 2019 (Acción Estratégica en Salud 2017-2020) to MP; Grant “CB16/11/00494” from CIBER-CV (CB16/11/00494) and Ayuda Grupos de Investigación de Extremadura (GR18199) from Consejería de Economía, Ciencia y Agenda Digital (cofunded by European Regional Development Fund – ERDF) to FS-M; a Postgraduate Scholarship for Foreign Studies (Fundación Ramón Areces, XXXI Edition) to MG-S; grants of the Deutsche Forschungsgemeinschaft (KFO325, 1408/14-1, and GRK2573/1) to EP; JV received funding from CIBER-CV (grant “CB16/11/00277”); Spanish Ministry of Science, Innovation and Universities (grants “BIO2015-67580-P” and “PGC2018-097019-B-I00”), ISCIII – Fondo de Investigación Sanitaria (PRB3 grant “IPT17/0019-ISCIII-SGEFI/ERDF”, ProteoRed), Fundació Marató TV3 (grant “122/C/2015”), and “la Caixa” Banking Foundation (project “HR17-00247”); JC received funding by ISCIII through a “Miguel Servet I” grant “MS17/00021” [co-funded by ERDF/European Social Fund (ESF) “A way to make Europe”/“Investing in your future”], the projects “CP17/00021” and “PI18/0911” (co-funded by ERDF/ESF), and by Junta de Extremadura through a “IB16168” grant (co-funded by ERDF/ESF). CNIC is supported by Instituto de Salud Carlos III (ISCIII), Ministerio de Ciencia, Innovación y Universidades (MCNU) and the Pro CNIC Foundation, and it is a Severo Ochoa Center of Excellence (SEV-2015-0505). Cell culture and in vitro studies were performed at the ICTS Nanbiosis (Unit 14, Stem Cell Therapy).es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Media es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleThe Immunomodulatory Signature of Extracellular Vesicles From Cardiosphere-Derived Cells: A Proteomic and miRNA Profiling.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32582685es_ES
dc.format.volume8es_ES
dc.format.page321es_ES
dc.identifier.doi10.3389/fcell.2020.00321es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderComunidad de Madrid (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderCentro de Investigación Biomedica en Red - CIBER
dc.contributor.funderFundación La Marató TV3 
dc.contributor.funderFundación La Caixa 
dc.contributor.funderFundación ProCNIC 
dc.contributor.funderGovernment of Extremadura (España) 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.3389/fcell.2020.00321es_ES
dc.identifier.journalFrontiers in cell and developmental biologyes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CD19/00048es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/FI19/00041es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CB16/11/00494es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CB16/11/00277es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MS17/00021es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2015-67580-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PGC2018-097019-B-I00es_ES
dc.rights.accessRightsopen accesses_ES


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