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dc.contributor.authorFernandez, Agustín F
dc.contributor.authorBayón, Gustavo F
dc.contributor.authorUrdinguio, Rocio G
dc.contributor.authorToraño, Estela G
dc.contributor.authorGarcía, María G
dc.contributor.authorCarella, Antonella
dc.contributor.authorPetrus-Reurer, Sandra
dc.contributor.authorFerrero, Cecilia
dc.contributor.authorMartinez-Camblor, Pablo
dc.contributor.authorDelgado-Calle, Jesús
dc.contributor.authorPérez-Campo, Flor M
dc.contributor.authorRiancho, José A
dc.contributor.authorBueno, Clara
dc.contributor.authorMenéndez, Pablo
dc.contributor.authorMentink, Anouk
dc.contributor.authorMareschi, Katia
dc.contributor.authorClaire, Fabian
dc.contributor.authorFagnani, Corrado
dc.contributor.authorMedda, Emanuela
dc.contributor.authorToccaceli, Virgilia
dc.contributor.authorBrescianini, Sonia
dc.contributor.authorMoran, Sebastián
dc.contributor.authorEsteller, Manel
dc.contributor.authorStolzing, Alexandra
dc.contributor.authorde Boer, Jan
dc.contributor.authorNisticò, Lorenza
dc.contributor.authorStazi, Maria A
dc.contributor.authorFraga, Mario F
dc.contributor.authorCubillo, Isabel 
dc.contributor.authorGarcia-Castro, Javier 
dc.date.accessioned2020-07-06T16:01:08Z
dc.date.available2020-07-06T16:01:08Z
dc.date.issued2015-01
dc.identifier.citationGenome Res . 2015 Jan;25(1):27-40.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10672
dc.description.abstractIn differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone post-translational modifications. However, the chromatin marks associated with changes in DNA methylation in adult stem cells during lifetime are still largely unknown. Here, DNA methylation profiling of mesenchymal stem cells (MSCs) obtained from individuals aged 2 to 92 yr identified 18,735 hypermethylated and 45,407 hypomethylated CpG sites associated with aging. As in differentiated cells, hypermethylated sequences were enriched in chromatin repressive marks. Most importantly, hypomethylated CpG sites were strongly enriched in the active chromatin mark H3K4me1 in stem and differentiated cells, suggesting this is a cell type-independent chromatin signature of DNA hypomethylation during aging. Analysis of scedasticity showed that interindividual variability of DNA methylation increased during aging in MSCs and differentiated cells, providing a new avenue for the identification of DNA methylation changes over time. DNA methylation profiling of genetically identical individuals showed that both the tendency of DNA methylation changes and scedasticity depended on nongenetic as well as genetic factors. Our results indicate that the dynamics of DNA methylation during aging depend on a complex mixture of factors that include the DNA sequence, cell type, and chromatin context involved and that, depending on the locus, the changes can be modulated by genetic and/or external factors.es_ES
dc.description.sponsorshipWe thank Ronnie Lendrum for manuscript preparation and Tim Triche Jr. for his invaluable advice. This work has been financially supported by the Plan Nacional de I+D+I 2008-2011/2013-2016/FEDER (PI11/01728 to A.F.F., PI 12/0615 to J.A.R., PI10/0449 to P.M., and PI11/0119 to C.B.); the ISCIII-Subdirección General de Evaluación y Fomento de la Investigación (Miguel Servet contracts CP11/00131 to A.F.F. and CP07/0059 to C.B.); the Spanish Ministry of Health (PS09/02454 and PI12/01080 to M.F.F.); the Spanish National Research Council (CSIC; 200820I172 to M.F.F.); IUOPA (to C.F. and G.F.B.); Fundacion Cientifica de la AECC (to R.G.U. and P.M.); Fundación Ramón Areces (to M.F.F.); and FICYT (to E.G.T.). J.G.-C. receives funding from the Fondo de Investigaciones Sanitarias (FIS; PI05/2217 and PI08/0029) and the Madrid Regional Government (S-BIO-0204-2006 and S2010/BMD-2420). J.A.R. receives funding from the Fondo de Investigaciones Sanitarias (ISCIII-FIS PI 12/0615). P.M. is also supported by MINECO (SAF2013/43065), ERANET E-Rare (PI112/03112), and Fundación Sandra Ibarra. P.M. also acknowledges support from Obra Social “La Caixa/Fundacio Josep Carreras.” The IUOPA is supported by the Obra Social Cajastur, Spain.es_ES
dc.language.isoenges_ES
dc.publisherCold Spring Harbor Laboratory Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshDNA Methylation es_ES
dc.subject.meshAdolescent es_ES
dc.subject.meshAged es_ES
dc.subject.meshAged, 80 and over es_ES
dc.subject.meshAging es_ES
dc.subject.meshCell Differentiation es_ES
dc.subject.meshCells, Cultured es_ES
dc.subject.meshChild es_ES
dc.subject.meshChild, Preschool es_ES
dc.subject.meshChromatin es_ES
dc.subject.meshDNA es_ES
dc.subject.meshEpigenesis, Genetices_ES
dc.subject.meshHistones es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMicroarray Analysis es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshPromoter Regions, Genetices_ES
dc.subject.meshProtein Processing, Post-Translationales_ES
dc.subject.meshSequence Analysis, DNA es_ES
dc.subject.meshStem Cells es_ES
dc.subject.meshTwins, Monozygotic es_ES
dc.subject.meshYoung Adult es_ES
dc.titleH3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID25271306es_ES
dc.format.volume25es_ES
dc.format.number1es_ES
dc.format.page27-40es_ES
dc.identifier.doi10.1101/gr.169011.113es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.contributor.funderFundación Científica AECCes_ES
dc.contributor.funderFundación Ramón Areceses_ES
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderFundación Sandra Ibarraes_ES
dc.contributor.funderFundación La Caixaes_ES
dc.contributor.funderFundación Jose Carrerases_ES
dc.contributor.funderFundación Cajastures_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1549-5469es_ES
dc.relation.publisherversionhttp://www.genome.org/cgi/doi/10.1101/gr.169011.113es_ES
dc.identifier.journalGenome researches_ES
dc.repisalud.centroISCIII::Instituto de Investigación de Enfermedades Rarases_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI11/01728es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ PI 12/0615es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI10/0449es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI11/0119es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CP11/00131es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CP07/0059es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PS09/02454es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI12/01080es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/200820I172es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI05/2217es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI08/0029es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/S-BIO-0204-2006es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/S2010/BMD-2420es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ISCIII-FIS PI 12/0615es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/SAF2013/43065es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI112/03112es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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