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dc.contributor.authorAguado-Urda, Mónica
dc.contributor.authorGibello, Alicia
dc.contributor.authorBlanco, M del Mar
dc.contributor.authorFernández-Garayzábal, José F
dc.contributor.authorLópez-Campos, Guillermo H
dc.contributor.authorBlanco, M. del Mar
dc.contributor.authorFernández-Garayzábal, José F.
dc.contributor.authorLópez-Campos, Guillermo H.
dc.contributor.authorLopez-Alonso, Victoria
dc.identifier.citationPLoS One . 2013 Nov 4;8(11):e79692.es_ES
dc.description.abstractLactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen.es_ES
dc.description.sponsorshipThis research was supported by grants AGL2009-12447 and AGL2012-35419 from the Spanish Ministry of Economy and Competiveness. MAU was recipient of a Ph.D. fellowship from the Complutense University of Madrid. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.subject.meshGene Expression Profiling es_ES
dc.subject.meshTemperature es_ES
dc.subject.meshLactococcus es_ES
dc.subject.meshOligonucleotide Array Sequence Analysis es_ES
dc.subject.meshTranscription, Genetic es_ES
dc.titleGlobal transcriptome analysis of Lactococcus garvieae strains in response to temperature.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderComplutense University of Madrid (España) 
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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Atribución 4.0 Internacional
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