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dc.contributor.authorPerdiguero, Eusebio
dc.contributor.authorMoiseeva, Victoria
dc.contributor.authorMunoz-Canoves, Pura 
dc.date.accessioned2020-06-29T11:30:00Z
dc.date.available2020-06-29T11:30:00Z
dc.date.issued2019-02
dc.identifier.citationMethods Mol Biol. 2019; 2045:13-23es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10606
dc.description.abstractThe maintenance of adult stem cells in their normal quiescent state depends on intrinsic factors and extrinsic signals originating from their microenvironment (also known as the stem cell niche). In skeletal muscle, its stem cells (satellite cells) lose their regenerative potential with aging, and this has been attributed, at least in part, to both age-associated changes in the satellite cells as in the niche cells, which include resident fibro-adipogenic progenitors (FAPs), macrophages, and endothelial cells, among others. To understand the regenerative decline of skeletal muscle with aging, there is a need for methods to specifically isolate stem and niche cells from resting muscle. Here we describe a fluorescence-activated cell sorting (FACS) protocol to simultaneously isolate discrete populations of satellite cells and niche cells from skeletal muscle of aging mice.es_ES
dc.description.sponsorshipWork in the authors’ laboratory has been supported by the Spanish Ministry of Science, Innovation and Universities, Spain (grant SAF2015-67369-R; and SAF 2015-70270-REDT, a María de Maeztu Unit of Excellence award to UPF [MDM-2014-0370], and a Severo Ochoa Center of Excellence award to the CNIC [SEV-2015-0505]), the UPF-CNIC collaboration agreement, ERC-2016-AdG-741966, La Caixa-HEALTH, AFM, MDA, and H2020-UPGRADE. V.M is recipient of a FPI predoctoral fellowship.es_ES
dc.language.isoenges_ES
dc.publisherHumana Press es_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshAdult Stem Cells es_ES
dc.subject.meshAging es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAntibodies es_ES
dc.subject.meshEndothelial Cells es_ES
dc.subject.meshFlow Cytometry es_ES
dc.subject.meshMacrophages es_ES
dc.subject.meshMesenchymal Stem Cells es_ES
dc.subject.meshMice es_ES
dc.subject.meshMuscle, Skeletal es_ES
dc.subject.meshReal-Time Polymerase Chain Reaction es_ES
dc.subject.meshRegeneration es_ES
dc.subject.meshSatellite Cells, Skeletal Muscle es_ES
dc.subject.meshStem Cell Niche es_ES
dc.subject.meshWorkflow es_ES
dc.titleSimultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID30771188es_ES
dc.format.volume2045es_ES
dc.format.page13-23es_ES
dc.identifier.doi10.1007/7651_2019_210es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC) 
dc.contributor.funderFundación La Caixa 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1940-6029es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/7651_2019_210es_ES
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)es_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Laboratorio de Regeneración Tisulares_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2015-67369-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2015-70270-REDTes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MDM-2014-0370es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/741966es_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional