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dc.contributor.authorGarcía-Prat, Laura
dc.contributor.authorMunoz-Canoves, Pura 
dc.contributor.authorMartínez-Vicente, Marta
dc.identifier.citationMethods Mol Biol. 2017; 1556:255-280es_ES
dc.description.abstractAutophagy is critical not only for the cell's adaptive response to starvation but also for cellular homeostasis, by acting as quality-control machinery for cytoplasmic components. This basal autophagic activity is particularly needed in postmitotic cells for survival maintenance. Recently, basal autophagic activity was reported in skeletal muscle stem cells (satellite cells) in their dormant quiescent state. Satellite cells are responsible for growth as well as for regeneration of muscle in response to stresses such as injury or disease. In the absence of stress, quiescence is the stem cell state of these cells throughout life, although which mechanisms maintain long-life quiescence remains largely unknown. Our recent findings showed that autophagy is necessary for quiescence maintenance in satellite cells and for retention of their regenerative functions. Importantly, damaged organelles and proteins accumulated in these cells with aging and this was connected to age-associated defective autophagy. Refueling of autophagy through genetic and pharmacological strategies restored aged satellite cell functions, and these finding have biomedical implications. In this chapter, we describe different experimental strategies to evaluate autophagic activity in satellite cells in resting muscle of mice. They should facilitate our competence to investigate stem cell functions both during tissue homeostasis as in pathological conditions.es_ES
dc.description.sponsorshipWork in the authors’ laboratories has been supported by: MINECO, Spain SAF2012-38547, AFM, E-Rare/Eranet, Fundació Marató-TV3, MDA, EU-FP7 (Myoage, Optistem, and Endostem), and DuchennePP-NL, to PM-C; and ISCIII-FEDER, Spain (FIS-PS09/01267, FIS-PI13/02512, CP09/00184, PI14/01529) and CIBERNED to MM-V. L.G.-P. was supported by a Predoctoral Fellowship from Programa de Formación de Personal Investigador (Spain).es_ES
dc.publisherHumana Press es_ES
dc.subject.meshAutophagy es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshBiological Assay es_ES
dc.subject.meshCell Separation es_ES
dc.subject.meshCellular Senescence es_ES
dc.subject.meshEnzyme Inhibitors es_ES
dc.subject.meshFlow Cytometry es_ES
dc.subject.meshFluorescent Antibody Technique es_ES
dc.subject.meshGene Expression es_ES
dc.subject.meshGene Expression Profiling es_ES
dc.subject.meshGenes, Reporter es_ES
dc.subject.meshMacrolides es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Transgenic es_ES
dc.subject.meshMicroscopy, Fluorescence es_ES
dc.subject.meshMicrotubule-Associated Proteins es_ES
dc.subject.meshMuscle, Skeletal es_ES
dc.subject.meshRecombinant Fusion Proteins es_ES
dc.subject.meshResting Phase, Cell Cycle es_ES
dc.subject.meshSatellite Cells, Skeletal Muscle es_ES
dc.subject.meshStem Cells es_ES
dc.subject.meshTranscriptome es_ES
dc.titleMonitoring Autophagy in Muscle Stem Cells.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderFundación La Marató TV3 
dc.contributor.funderUnión Europea 
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)es_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Laboratorio de Regeneración Tisulares_ES
dc.rights.accessRightsopen accesses_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional